Sökning: "ion chromatography"

Visar resultat 1 - 5 av 70 uppsatser innehållade orden ion chromatography.

  1. 1. Creating a High-Throughput Workflow for Automated Peptide Characterization using LC-MS

    Master-uppsats, Lunds universitet/Centrum för analys och syntes

    Författare :Sara Forsberg; [2023]
    Nyckelord :High-throughput analysis; Impurity profiling; UHPLC-HRMS; Peptide characterization; Peptide descriptors; Analytical chemistry; Technology and Engineering;

    Sammanfattning : In the early stage of a drug discovery project, there is a need for efficient methods that can analyse peptides in short time. This includes methods that confirm the peptide’s identity and estimates its relative purity in an efficient and reliable way. LÄS MER

  3. 2. Surface functionalization of cellulose fibers and exploration of its influence in ion exchange chromatography

    Master-uppsats, KTH/Fiber- och polymerteknologi

    Författare :Anna Wessén; [2023]
    Nyckelord :Ion-exchange chromatography; membrane chromatography; surface extension; grafting; copolymerization; free-radical polymerization; Jonbyteskromatografi; membrankromatografi; ytförlängning; ympning; sampolymerisation; fri radikal polymerisation;

    Sammanfattning : Kromatografiska metoder kan användas för en mängd olika tillämpningar, inklusive separation, rening och analys av biomolekyler och kemikalier. Membrankromatografi är en av de nya kromatografiteknikerna som expanderar snabbt eftersom den tillåter hög masstransport genom den stationära fasen, vilket resulterar i en mer tidseffektiv process. LÄS MER

  4. 3. Ion pair chromatography of carboxylic and sulfonic acids with trialkylamines : An investigation of the relationship between the adsorption isotherm of the IPR and the retention factor of the analyte

    Kandidat-uppsats, Karlstads universitet/Institutionen för ingenjörs- och kemivetenskaper (from 2013)

    Författare :Karl Kullberg; [2023]
    Nyckelord :;

    Sammanfattning : Ion pair chromatography has found many uses within analytical chemistry and understanding of the chromatographic process with an ion-pairing reagent have been studied since the 1970s. The adsorption of the Ion-pairing reagent to the stationary phase is understood to be important and many studies have been dedicated to looking at the adsorption isotherms of the earlier quaternary alkylammonium ions. LÄS MER

  5. 4. Exploratory study of ion pair liquid chromatography separation methods and sample preparation procedures – for quantification of amount 5’capped and uncapped mRNA

    Kandidat-uppsats, Karlstads universitet/Institutionen för ingenjörs- och kemivetenskaper (from 2013)

    Författare :Felicia Lindström; [2023]
    Nyckelord :;

    Sammanfattning : Using ion pair chromatography, separation and analysis of shorter mRNA fragments was possible. The aim of the study was to develop a separation method for quantitation of 5’capped versus uncapped mRNA. Cleavage of mRNA was performed by complementary probes and the enzyme RNase H which created shorter mRNA fragments containing the 5’ end. LÄS MER

  7. 5. Towards time-resolved cryo-EM of SARS-CoV-2 replication-transcription complex and Staphylococcus aureus DNA gyrase

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Anna Králová; [2023]
    Nyckelord :time-resolved cryo-EM; DNA gyrase; SARS-CoV-2; replication-transcription complex; protein purification;

    Sammanfattning : Time-resolved cryo-EM has already provided ground-breaking discoveries in various fields, including structural biology, biochemistry, and drug development. Compared to traditional structural biology methods where mostly stabilized conformations are reconstructed, the main advantage of time-resolved cryo-EM is its ability to capture dynamic processes in biological samples at near-atomic resolution, which allows for studying biological structures as they change and interact in real-time. LÄS MER