Sökning: "keratinase"

Hittade 3 uppsatser innehållade ordet keratinase.

  1. 1. Optimization of growth conditions for a recombinant keratinase producing bacterial strain

    Master-uppsats, Högskolan i Borås/Institutionen Ingenjörshögskolan

    Författare :Kristina Laurila; [2014]
    Nyckelord :Keratinase; chicken feathers; growth; Bacillus megaterium;

    Sammanfattning : A recombinant Bacillus megaterium strain with a chromosomally integrated gene kerA, expressing keratinase, was analyzed regarding action in specific environmental stresses to obtain optimum parameters necessary for bacterial growth and optimal expression of the recombinant gene.The strain was grown on chicken feathers as substrate and the degradation of feather keratin during the growth was investigated with different levels of pH, temperature and feather concentration in the broth. LÄS MER

  3. 2. Chromosomal Integration of KerA Gene in Bacillus megaterium For Stable Keratinase Production

    Magister-uppsats, Högskolan i Borås/Institutionen Ingenjörshögskolan

    Författare :Eniyan Jalendran; Seyed Javad Dadvar Baygi; [2011]
    Nyckelord :bacillus lichiniformis; chromosomal integration; bacillus megaterium; integration vector; pmutin gfp 6192; keratinase;

    Sammanfattning : In order to develop a stable strain of Bacillus megaterium for Keratinase production, the Keratinase gene (KerA) of Bacillus lichiniformis ATCC 53757 and SPlipA gene from plasmid pHIS1525.SPlipA (Bacillus megaterium origin) were PCR amplified and constructed to give a gene cassette called SPK . LÄS MER

  4. 3. Production of recombinant keratinase for poultry feather degradation

    Magister-uppsats, Högskolan i Borås/Institutionen Ingenjörshögskolan

    Författare :Saeid Alinezhad; Amir Mirabdollah; [2009]
    Nyckelord :recombinant keratinase; bacillus megaterium; feathers degradation; expression vector;

    Sammanfattning : The keratinase gene (kerA) of Bacillus licheniformis ATCC®53757 was PCR amplified and subsequently cloned into Bacillus megaterium expression vector; pHIS1525.SPlipA and transformed in Bacillus megaterium ATCC®14945. The kerA gene carrying the recombinant plasmid pKERHIS1525. LÄS MER