Sökning: "kloning"

Visar resultat 1 - 5 av 21 uppsatser innehållade ordet kloning.

  1. 1. Security evaluation of a smart lock system

    M1-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Författare :Raihana Hassani; [2020]
    Nyckelord :Cyber security; Threat modeling; Penetration testing; Ethical hacking; Smart locks; STRIDE; DREAD; Cybersäkerhet; Hotmodellering; Penetrationstestning; Etisk hackning; Smarta lås; STRIDE; DREAD;

    Sammanfattning : Cyber attacks are an increasing problem in the society today. They increase dramatically, especially on IoT products, such as smart locks. This project aims to evaluate the security of the Verisure smartlock system in hopes of contributing to a safer development of IoT products and highlighting theexisting flaws of today’s society. LÄS MER

  2. 2. Kloning, överuttryck och upprening av växtakvaporinet AtSIP1;1 i Escherichia coli

    Kandidat-uppsats, Lunds universitet/Examensarbeten i molekylärbiologi

    Författare :Johan Zhu; [2020]
    Nyckelord :Biology and Life Sciences;

    Sammanfattning : Heterologous overexpression of proteins signifies that the encoding gene of a protein in one organism is expressed in another host organism that normally does not have that gene in its genome. In basic biochemical research, this is an important method for studying various protein functions such as activity and interactions in biological processes, as well as obtaining sufficient amounts to determine protein structures. LÄS MER

  3. 3. Production and validation of anti-HCV antibodies for viral neutralization

    Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Författare :Megan Olga García Mark; [2020]
    Nyckelord :Cloning; antibody; infection; HCV; IgG production; molecular biology; cell factory; plasmid design; immunology;

    Sammanfattning : Hepatitis-C Virus (HCV) remains the leading cause of liver transplant in the US and the UK, and the World Health Organization (WHO) estimates that 71 million people are infected worldwide. A vaccine would drastically impact the healthcare-associated burdens that HCV causes globally. LÄS MER

  4. 4. Imitation Learning using Reward-Guided DAgger

    Master-uppsats, KTH/Skolan för elektroteknik och datavetenskap (EECS)

    Författare :Nora Al-Naami; [2020]
    Nyckelord :;

    Sammanfattning : End-to-end autonomous driving can be approached by finding a policy function that maps observation (e.g. driving view of the road) to driving action. This is done by imitating an expert driver. LÄS MER

  5. 5. Production of gangliosidebiosynthetic membrane enzymes for biochemical and functionalstudies : Expression, purification and crystallizationoptimization of Thermococcus onnurineus Dolicho l-phosphate mannose synthase, Homosapiens and Branchiostoma floridae Glucosylceramide synthase

    Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Författare :Ellinor Lindholm; [2018]
    Nyckelord :glycoconjugates; glycolipids; integral membrane proteins; gangliosides; glucosylceramide synthase; dolicholphosphate mannose synthase; cancer; neuroblastoma; congenital disorders of glycosylation; glykokonjukat; glykolipider; membranprotein; gangliosider; glukosylceramidsyntas; dolicholfosfat mannossyntas; cancer; neuroblastom; medfdd glykosyleringssjukdom;

    Sammanfattning : Glycolipids play important roles in the biology of prokaryotes and eukaryotes, including humans, and although theyare found on the cell-membrane surface of all eukaryotic cells, not much is known about their biosynthesis. The aim ofthis project was to characterize two enzymes: glucosylceramide synthase (GCS) which is involved in the biosynthesisof glycolipids such as gangliosides that are abundant in the membranes of nerve cells; and dolicholphosphate mannosesynthase (DPMS), involved in the synthesis precursor for protein glycosylation. LÄS MER