Sökning: "kloning"

Visar resultat 6 - 10 av 26 uppsatser innehållade ordet kloning.

1. 6. Kloning, överuttryck och upprening av växtakvaporinet AtSIP1;1 i Escherichia coli

   Kandidat-uppsats, Lunds universitet/Examensarbeten i molekylärbiologi

   Författare :Johan Zhu; [2020]
   Nyckelord :Biology and Life Sciences;

   Sammanfattning : Heterologous overexpression of proteins signifies that the encoding gene of a protein in one organism is expressed in another host organism that normally does not have that gene in its genome. In basic biochemical research, this is an important method for studying various protein functions such as activity and interactions in biological processes, as well as obtaining sufficient amounts to determine protein structures. LÄS MER

3. 7. Production and validation of anti-HCV antibodies for viral neutralization

   Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

   Författare :Megan Olga García Mark; [2020]
   Nyckelord :Cloning; antibody; infection; HCV; IgG production; molecular biology; cell factory; plasmid design; immunology;

   Sammanfattning : Hepatitis-C Virus (HCV) remains the leading cause of liver transplant in the US and the UK, and the World Health Organization (WHO) estimates that 71 million people are infected worldwide. A vaccine would drastically impact the healthcare-associated burdens that HCV causes globally. LÄS MER

4. 8. Imitation Learning using Reward-Guided DAgger

   Master-uppsats, KTH/Skolan för elektroteknik och datavetenskap (EECS)

   Författare :Nora Al-Naami; [2020]
   Nyckelord :;

   Sammanfattning : End-to-end autonomous driving can be approached by finding a policy function that maps observation (e.g. driving view of the road) to driving action. This is done by imitating an expert driver. LÄS MER

5. 9. Identification and Expression of Oat Hemoglobin

   Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

   Författare :Simon Christensen; [2019]
   Nyckelord :Hemoglobin; Oat; Plant Hemoglobin; Hemoglobin-based oxygen carrier; non-symbiotic Hemoglobin; oxygen transporter; applied biochemistry; Biology and Life Sciences; Technology and Engineering; Medicine and Health Sciences;

   Sammanfattning : Hemoglobin is mainly viewed as an oxygen and carbon dioxide carrier in the blood of animals and thus essential for life as we know it. During the course of evolution, plants have also developed versions of this protein, for similar and different purposes, such as regulation of nitric oxide levels. LÄS MER

7. 10. Production of gangliosidebiosynthetic membrane enzymes for biochemical and functionalstudies : Expression, purification and crystallizationoptimization of Thermococcus onnurineus Dolicho l-phosphate mannose synthase, Homosapiens and Branchiostoma floridae Glucosylceramide synthase

   Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

   Författare :Ellinor Lindholm; [2018]
   Nyckelord :glycoconjugates; glycolipids; integral membrane proteins; gangliosides; glucosylceramide synthase; dolicholphosphate mannose synthase; cancer; neuroblastoma; congenital disorders of glycosylation; glykokonjukat; glykolipider; membranprotein; gangliosider; glukosylceramidsyntas; dolicholfosfat mannossyntas; cancer; neuroblastom; medfdd glykosyleringssjukdom;

   Sammanfattning : Glycolipids play important roles in the biology of prokaryotes and eukaryotes, including humans, and although theyare found on the cell-membrane surface of all eukaryotic cells, not much is known about their biosynthesis. The aim ofthis project was to characterize two enzymes: glucosylceramide synthase (GCS) which is involved in the biosynthesisof glycolipids such as gangliosides that are abundant in the membranes of nerve cells; and dolicholphosphate mannosesynthase (DPMS), involved in the synthesis precursor for protein glycosylation. LÄS MER