Sökning: "kryo-elektronmikroskopi"

Hittade 3 uppsatser innehållade ordet kryo-elektronmikroskopi.

  1. 1. Imaging of the Cytosolic Antibody Receptor TRIM21

    Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Författare :Þórunn Stefánsdóttir; [2022]
    Nyckelord :TRIM21; ProteinStructure; Antibody; cryo-EM; Gel-Filtration SizeExclusion Chromatography ; Immunology; ProteinPurification; SDS-PAGE; TRIM21; Proteinstruktur; Antikroppar; cryo-EM; Gel-filtreringsKromatografi; Immunologi; ProteinRening; SDS-PAGE;

    Sammanfattning : TRIM21 is a cytosolic ubiquitin ligase and an antibody receptor that providesa last line of defense against invading pathogens. By utilizing the diversity ofantibody repertoire to identify pathogens, TRIM21 serves as a link betweenintrinsic cellular defense and adaptive immunity. LÄS MER

  3. 2. Design, production and evaluation of cross linked target proteins to an affibody-based carrier framework aimed for affinity protein: antigen structure determination using single particle Cryo-EM

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Richard Brunsell; [2021]
    Nyckelord :Cryo-EM; Structure determination; Protein A; Affibody; Aldolase; Scaffold; Rigid helix fusion; Photoconjugation; UV crosslinking; BPA;

    Sammanfattning : Small proteins are difficult to study at high resolution with single-particle cryo-electron microscopy (cryo-EM). In general, sample properties such as large size (> 80 kDa), symmetry and rigidity are key to utilize this technology. LÄS MER

  4. 3. Structural basis of modulation by pH and calcium in a ligand-gated ion channel

    Master-uppsats, KTH/Genteknologi

    Författare :Olivia Andén; [2021]
    Nyckelord :Pentameric ligand-gated ion channels; bacterial homolog; protein expression; protein purification; cryo-electron microscopy; data processing; protein structure; Pentamerisk ligandstyrd jonkanal; bakteriell homolog; proteinexpression; proteinrening; kryo-elektronmikroskopi;

    Sammanfattning : Pentameriska ligandstyrda jonkanaler (pLGICs) är avgörande för omvandlingen av kemisk till elektrisk signalöverföring i djurs nervsystem. Dysfunktion i dessa kanaler har visat sig vara kopplad till flera sjukdomar inklusive epilepsi, schizofreni, Alzheimers och autism, vilket gör dem till en måltavla för en mängd olika läkemedel. LÄS MER