Sökning: "liquid chromatography tandem mass spectrometry LC-MS MS"

Visar resultat 1 - 5 av 19 uppsatser innehållade orden liquid chromatography tandem mass spectrometry LC-MS MS.

1. 1. A Comparative Analysis of Per- andPolyfluoroalkyl Substances (PFAS) and ExtractableOrganofluorine (EOF) Using Solid PhaseExtraction-Weak Anion Exchange and Ion PairExtraction in SerumMarichal SalamehSpring 2021Independent project

   Kandidat-uppsats, Örebro universitet/Institutionen för naturvetenskap och teknik

   Författare :Marichal Salameh; [2021]
   Nyckelord :Extractable organofluorine EOF ; Per- and polyfluoroalkyl substances PFAS ; horse serum; human serum; ion pair extraction IPE ; solid phase extraction - weak anion exchange SPE-WAX ; liquid chromatography tandem mass spectrometry LC-MS MS ; combustion ion chromatography CIC ;

   Sammanfattning : Per- and polyfluorinated substances (PFAS) are compounds that consist of a carbon chainbackbone that is partially or entirely fluorinated, with an addition of a functional group. SomePFAS are known as persistent organic pollutants (POPs) and have therefore been drawing a lot ofattention as well as increased concerns. LÄS MER

3. 2. Detektion av hydrolyserad β-laktamantibiotika i plasma med Matrix-Assisted Laser Desorption Ionization – Time of Flight Mass Spectrometry och Liquid Chromatography tandem Mass Spectrometry

   Kandidat-uppsats, Örebro universitet/Institutionen för hälsovetenskaper

   Författare :Niklas Thenstedt; [2020]
   Nyckelord :LC-MS MS; MALDI-TOF MS; Enterobacteriaceae; sepsis; piperacillin-tazobactam; cefotaxime; Extended spectrum β-lactamase; hydrolysis; LC-MS MS; MALDI-TOF MS; Enterobacteriaceae; sepsis; piperacillin-tazobaktam; cefotaxim; Extended spektrum β-laktamas; hydrolys;

   Sammanfattning : Introduktion Antibiotikaresistens är ett globalt växande problem. Till gruppen β-laktamantibiotika hör piperacillin-tazobaktam och cefotaxim som båda verkar genom att försvaga cellväggen med kovalenta bindningar till peptidoglykanlagret som lyserar cellen. E. coli och K. LÄS MER

4. 3. Development and validation of an ultrafiltration-UHPLC-MS/MS method for the quantification of unbound Beta-Lactam antibiotics cefotaxime, piperacillin, cloxacillin and flucloxacillin in plasma

   Master-uppsats, KTH/Tillämpad fysikalisk kemi

   Författare :Leona Clarin; [2020]
   Nyckelord :Unbound concentration; free concentration; ultrafiltration; LC-MS MS; cefotaxime; flucloxacillin; cloxacillin; piperacillin; Fri fraktion; ultrafiltrering; LC-MS MS; cefotaxim; flukloxacillin; kloxacillin; piperacillin;

   Sammanfattning : Infections in critically ill patients are a problem for the healthcare system and at any one time, 70 % of all intensive care unit (ICU) patients are treated with antibiotics. Antibiotics bind toproteins in the blood, but only unbound drug can diffuse over capillary membranes and bindto the targeted receptor. LÄS MER

5. 4. Identification of metabolite-protein interactions among enzymes of the Calvin Cycle in a CO2-fixing bacterium

   Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

   Författare :Emil Sporre; [2020]
   Nyckelord :Cupriavidus necator; chemolithoautotroph; limited proteolysis; Calvin Cycle; carbon fixation; proteomics; allosteric regulation; metabolite-protein interactions.;

   Sammanfattning : The Calvin – Benson cycle is the most widespread metabolic pathway capable of fixing CO2 in nature and a target of very high interest to metabolic engineers worldwide. In this study, 12 metabolites (ATP, AMP, NADP, NADPH, 2PG, 3PGA, FBP, RuBP, PEP, AKG, Ac-CoA and phenylalanine) were tested for protein – metabolite interactions against the proteome of Cupriavidus necator (previously Ralstonia eutropha) in the hopes of finding potential examples of allosteric regulation of the Calvin – Benson cycle. LÄS MER

7. 5. Total oxidizable precursor assay: Applications and limitations. A study on the occurrence of perfluoroalkyl and polyfluoroalkyl substances (PFASs) in Chinese Wastewater Treatment Plants

   Master-uppsats, Örebro universitet/Institutionen för naturvetenskap och teknik

   Författare :Pontus Larsson; [2020]
   Nyckelord :;

   Sammanfattning : Total oxidizable precursor (TOP) assay is an in-lab method which oxidatively converts precursor compounds of poly- and perfluoroalkyl substances (PFASs) into measurable perfluorinated alkyl acids (PFAAs). The method is a very strong tool to semi-quantify PFASs that would otherwise be missed in conventional targeted analysis using liquid chromatography - tandem mass spectrometry (LC-MS/MS). LÄS MER