Sökning: "mRNA extraction"

Hittade 3 uppsatser innehållade orden mRNA extraction.

1. 1. Investigation of Chromatin Organization and mRNA Expression in Drug Treated Human Erythroleukemia Cells

   Master-uppsats, KTH/Proteinvetenskap

   Författare :Anam Minhas; [2022]
   Nyckelord :Erythroleukemia cells; mRNA-seq; ATAC-seq; Doxorubicin; Vinblastine; Erytroleukemiceller; mRNA-expression; kromatin; Doxorubicin; Vinblastine;

   Sammanfattning : Syftet med detta projekt var att undersöka hur vanligt använda cancerläkemedel påverkar mRNA-uttryck och kromatinorganisation i humana erytroleukemiceller. Som modell användes K562-celler från en patient i blastocystkris (2), för att utvärdera leukemicellernas svar på cancerläkemedel vinblastin och doxorubicin. LÄS MER

3. 2. Development and evaluation of a pre-analytical device for liquid biopsy

   Uppsats för yrkesexamina på avancerad nivå,

   Författare :Nicole Yacoub; [2018]
   Nyckelord :;

   Sammanfattning : In this project, a novel method to discover and monitor cancer was studied. Tumoureducated platelets (TEPs) have shown the ability to take up tumour-derived secretedmembrane vesicles, which contain tumour RNA. Therefore they are of great interestfor detecting cancer and could potentially work as biomarkers. LÄS MER

4. 3. Optimization of a specific messenger RNA extraction protocol for fresh and vitrified bovine oocytes to gene expression studies : Specific mRNA extraction protocol for bovine oocytes.

   Magister-uppsats,

   Författare :Krishna Chaitanya Pavani; [2012]
   Nyckelord :mRNA extraction ; bovine embryos; gene expression; modified protocol extraction ;

   Sammanfattning : To understand bovine oocytes meiotic maturation, developmental potential, gene expression is required. The gene expression studies in the preimplantation bovine oocytes has been difficult, because the procedures that are being employed for extracting total RNA are not specific for bovine oocytes and so far is not providing the required amount for further procedures. LÄS MER