Sökning: "metal-binding"

Hittade 4 uppsatser innehållade ordet metal-binding.

  1. 1. Investigation of the function of the SH3-like domain of the Saccharopolyspora erythraea iron-dependent regulator

    Master-uppsats, Uppsala universitet/Institutionen för kemi - BMC

    Författare :Carita Hammar; [2022]
    Nyckelord :;

    Sammanfattning : The bacterium Saccharopolyspora erythraea is utilized to produce the widely used antibiotic erythromycin. With the increasing problem of antibiotic resistance, the development of derivatives of the drug is of great importance. To produce antibiotics biosynthetically, it is crucial to understand the metabolic pathways utilized by the bacterium. LÄS MER

  3. 2. Purification, functional characterization and crystallization of the PerR peroxide sensor from Saccharopolyspora erythraea

    Master-uppsats, Uppsala universitet/Strukturbiologi

    Författare :Grim Elison Kalman; [2019]
    Nyckelord :structural biology; cell culture; protein expression; genetic engineering; spectroscopy; X-ray crystallography; small angle X-ray scattering; SAXS; transcription factor; PerR; metal binding; antibiotics; Saccharopolyspora erythraea; S. erythraea; soil bacterium; actinobacteria; actinomycetes; oxidative stress; peroxide stress; structure determination; characterization; stabilization; peroxide sensor; iron sensor; manganese sensor; metalloprotein; metal ion homeostasis; metal ion; prokaryotic; strukturbiologi; cellodling; proteinuttryck; genteknik; spektroskopi; röntgenkristallografi; småvinkel-röntgenspridning; SAXS; transkriptionsfaktor; PerR; metallbindande; antibiotika; Saccharopolyspora erythraea; S. erythraea; jordartsbakterie; Aktinobakterier; Actinobacteria; Actinomycetes; oxidativ stress; peroxid stress; strukturbestämning; karaktärisering; stabilisering; peroxidsensor; järnsensor; mangansensor; metalljonshomeostas; metalljoner; prokaryot;

    Sammanfattning : This report summarizes the work on the cloning, expression, and purification of PerR, a metal sensing regulator from Saccharopolyspora erythraea and the subsequent characterization using small angle X-ray scattering and other biochemical methods. The report aims to provide an insight into prokaryotic metal homeostasis, provide a better understanding of how PerR works and provide valuable information for the continued work on the crystallization of PerR. LÄS MER

  4. 3. Understanding the Involvement of Leukocyte Cell-derived Chemotaxin 2 (LECT2) in Amyloidosis

    Master-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

    Författare :Lisa-Marie Erlandsson; [2019]
    Nyckelord :LECT2 amyloidosis; disulfide bonds; metal-binding; protein expression; refolding; purification; fluorimetry; CD; aggregation;

    Sammanfattning : Leukocyte cell-derived chemotaxin 2 (LECT2) is a zinc-binding multi-functional protein comprising three disulfide bonds, that is involved in multiple disorders of worldwide concern. Recently LECT2 was found to be involved in amyloidosis (ALECT2) and is believed to be the third most common form of systemic amyloidosis. LÄS MER

  5. 4. The metal binding properties of kraft lignin

    Uppsats för yrkesexamina på avancerad nivå, Akademin för hållbar samhälls- och teknikutveckling; Institutionen för biologi och kemiteknik

    Författare :Johanna Waltersson; [2009]
    Nyckelord :Lignin;

    Sammanfattning : There is a strong driving force to increase the competitiveness of the pulping industry by finding new business opportunities. In this context full utilisation of the wood raw material used in conventional pulping mills is of vital importance. One focus area is to increase the utilisation areas of lignin. LÄS MER