Sökning: "metallbindande"

Hittade 1 uppsats innehållade ordet metallbindande.

1. 1. Purification, functional characterization and crystallization of the PerR peroxide sensor from Saccharopolyspora erythraea

   Master-uppsats, Uppsala universitet/Strukturbiologi

   Författare :Grim Elison Kalman; [2019]
   Nyckelord :structural biology; cell culture; protein expression; genetic engineering; spectroscopy; X-ray crystallography; small angle X-ray scattering; SAXS; transcription factor; PerR; metal binding; antibiotics; Saccharopolyspora erythraea; S. erythraea; soil bacterium; actinobacteria; actinomycetes; oxidative stress; peroxide stress; structure determination; characterization; stabilization; peroxide sensor; iron sensor; manganese sensor; metalloprotein; metal ion homeostasis; metal ion; prokaryotic; strukturbiologi; cellodling; proteinuttryck; genteknik; spektroskopi; röntgenkristallografi; småvinkel-röntgenspridning; SAXS; transkriptionsfaktor; PerR; metallbindande; antibiotika; Saccharopolyspora erythraea; S. erythraea; jordartsbakterie; Aktinobakterier; Actinobacteria; Actinomycetes; oxidativ stress; peroxid stress; strukturbestämning; karaktärisering; stabilisering; peroxidsensor; järnsensor; mangansensor; metalljonshomeostas; metalljoner; prokaryot;

   Sammanfattning : This report summarizes the work on the cloning, expression, and purification of PerR, a metal sensing regulator from Saccharopolyspora erythraea and the subsequent characterization using small angle X-ray scattering and other biochemical methods. The report aims to provide an insight into prokaryotic metal homeostasis, provide a better understanding of how PerR works and provide valuable information for the continued work on the crystallization of PerR. LÄS MER