Sökning: "minicircle"
Hittade 2 uppsatser innehållade ordet minicircle.
1. Metoder som minskar bildning av teratom vid in vivo transplantation av inducerade pluripotenta celler
Kandidat-uppsats, SLU/Dept. of Biomedical Sciences and Veterinary Public HealthSammanfattning : Inducerad pluripotens innebär att man inducerar mogna celler till att övergå i ett pluripotent stadium och bilda så kallade inducerade pluripotenta celler (iPS-celler). Detta görs till exempel genom viral integration av transkriptionsfaktorerna octamer-binding transcription factor 3 och 4 (Oct3/4), sex determining region Y-box 2 (Sox2), Krüppel-like factor 4 (Klf4) och c-Myc. LÄS MER
2. Rolling circle transcription on smallest size double stranded DNA minicircles
Master-uppsats, Institutionen för biologisk grundutbildningSammanfattning : The RNA polymerase T7 is utilized as a component of motor complexes in DNA nanotechnology due to its high promotor specificity, the lack of external transcription factors and its very high processivity, but there is no experience of its application on small double stranded DNA circles. Circular templates from 210 to 126 bp in circumference sharing a common promotor termination motif were synthesized and transcription was monitored at end point on gel and in real time with a 2’ O methyl RNA molecular beacon. LÄS MER