Sökning: "mixed mode column"

Hittade 4 uppsatser innehållade orden mixed mode column.

  1. 1. Investigation of Adsorption and Retention of Charged Compounds In RPLC

    Kandidat-uppsats, Karlstads universitet/Institutionen för ingenjörs- och kemivetenskaper (from 2013)

    Författare :Emma Fryxelius; [2022]
    Nyckelord :adsorption; adsorption isotherm; charged compounds; promethazine hydrochloride; propranolol hydrochloride; mixed-mode; retention; RPLC;

    Sammanfattning : The adsorption isotherm of two weak bases, Promethazine hydrochloride and Propranolol hydrochloride, were determined with isocratic reversed-phase liquid chromatography, with a 60 w% methanol in 20 mM sodium acetate buffer pH 4 as the mobile phase, and calculated by the elution by characteristic points method. The data obtained from the method were then fitted into the Langmuir isotherm and the electrostatically modified Langmuir. LÄS MER

  3. 2. Oligonucleotide analysis with 2D-LC-MS using mixed mode column

    Master-uppsats, Uppsala universitet/Analytisk farmaceutisk kemi

    Författare :Esther Goldberg; [2019]
    Nyckelord :mass spectrometry; 2D-LC-MS; mixed mode column; oligonuecleotide;

    Sammanfattning : In this project method development for analysis and characterization of an oligonucleotide sample containing two major impurities was done. For this purpose UPLC was used with a RP-WAX mixed mode column with UV and/or MS detection. LÄS MER

  4. 3. Development of separation method for analysis of oligonucleotides using LC-UV/MS

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Analytisk farmaceutisk kemi

    Författare :Björs Ida; [2018]
    Nyckelord :Oligonucleotides; UPLC; liquid chromatography; time of flight; mass spectrometry; reversed-phase weak anion exchange; AcclaimTM Mixed Mode WAX-1; DNAPacTM RP; ACQUITY UPLC BEH C18;

    Sammanfattning : Introduction Oligonucleotides are short nucleic acid chains, usually 19-27mer long. They bind to their corresponding chain, making a specific inhibition possible. In pharmaceuticals, this can be used to inhibit the expression of a gene or protein of interest. LÄS MER

  5. 4. Utveckling av HPLC-metoder för kvantifiering av nyckelkomponenter i en villkorad emulsion

    Kandidat-uppsats, Institutionen för fysik, kemi och biologi

    Författare :Mikael Persson; [2009]
    Nyckelord :ELSD; HPLC; mixed-mode kolonn; polyglykol; dikarboxylsyra;

    Sammanfattning : Traditionally, rolling mills use emulsions based on a mixture of oil and water for lubrication. Since two years ago SAPA has been using (instead of oil) a synthetic lubricant so called conditional emulsion for hot-rolling of aluminum. LÄS MER