Sökning: "optimizing protein purification"

Hittade 4 uppsatser innehållade orden optimizing protein purification.

  1. 1. Optimizing the Production of Aurora Kinase A and Validation of Constructs with different Sequential Lengths

    Master-uppsats, Linköpings universitet/Kemi

    Författare :Linnea Pierre; [2022]
    Nyckelord :Protein; Protein chemistry; Aurora Kinase A; Aurora A; Cancer; Structural Biology; Cancer research;

    Sammanfattning : Aurora Kinase A is a kinase involved in multiple signaling pathways and interactions during mitosis, making it an essential kinase that deregulated causes cancer diseases in affected patients. Structural research shows mainly static snapshots of possible conformations of the partly disordered protein. LÄS MER

  3. 2. Production and characterisation of a chlamydial antigen candidate for vaccine trials

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Molekylär biomimetik

    Författare :Therese Koivula; [2021]
    Nyckelord :Chlamydia trachomatis; Immunology; Vaccine; Optimizing protein expression in E. coli; Optimizing protein purification; IMAC; His-tag cleavage; Buffer exchange; Chlamydial antigen;

    Sammanfattning : The bacterium Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infection worldwide. When left untreated, chlamydial infections can lead to severe complications, such as infertility. LÄS MER

  4. 3. CUSTOMIZED QUANTIFICATION OF HOST CELL PROTEIN WITH BIO-LAYER INTERFEROMETRY

    Master-uppsats, Lunds universitet/Teknisk mikrobiologi

    Författare :Eva Sjöblom; [2020]
    Nyckelord :BLI; HCP; CHO; yeast; host cell protein; bio-layer interferometry; immunoassays; quantification; chinese hamster ovary; ELISA; applied microbiology; teknisk mikrobiologi; Biology and Life Sciences; Technology and Engineering;

    Sammanfattning : Host cell protein (HCP) is quantified in the purification steps of biopharmaceutical production and is part of ensuring the purity of the final drug product. The gold standard method for HCP quantification is enzyme-linked immunosorbent assay (ELISA), but a new approach is proposed here with fully customized bio-layer interferometry assays for yeast and chinese hamster ovary (CHO) cells. LÄS MER

  5. 4. DEVELOPING A MOLECULAR TOOL KIT FOR DIAGNOSTIC PCR

    Uppsats för yrkesexamina på grundnivå, Uppsala universitet/Institutionen för kvinnors och barns hälsa

    Författare :Neima Mohamed Moumin; [2019]
    Nyckelord :Taq polymerase; Taq purification; Recombinant protein production; Real-Time PCR; Gel electrophoresis;

    Sammanfattning : ABSTRACT The aim of this study is develop and test an inexpensive molecular tool kit to be used for diagnostic PCR for diseases such as Leber hereditary optic neuropathy (LHON) and Cystic fibrosis(CF). By developing and optimizing recombinant Taq polymerase and making a DNA size ladder from plasmids pPSU1 and pPSU2 the financial cost for the tool kit would be reduced significantly compared to the commercial components. LÄS MER