Sökning: "ouabain"

Hittade 5 uppsatser innehållade ordet ouabain.

  1. 1. Development and Application of Analytical Tools for Calcium Signaling Dynamics in MDCK-II Cells

    Master-uppsats, KTH/Tillämpad fysik

    Författare :Hanna Israelsson; [2023]
    Nyckelord :Cellpose 2.0; MDCKII; GCaMP6m; Gap Junctions; Calcium; Ouabain; Cellpose 2.0; MDCKII; GCaMP6m; Gap Junctions; Kalcium; Ouabain;

    Sammanfattning : Calcium, a pivotal ion in various human signaling pathways, holds significant relevance in cancer research and treatment. Investigating the transmission of spontaneous calcium signals between cells and understanding how they are influenced by different treatments is therefore an interesting area of study. LÄS MER

  3. 2. The role of gap junctions in coordination of intercellular Ca2+ signaling

    Master-uppsats, KTH/Tillämpad fysik

    Författare :Emma Latron; [2023]
    Nyckelord :applied physics; biophysics; Calcium; MDCKII; Gap Junctions; signalling; Segmentation; Tillämpad fysik; Biofysik; Kalcium; MDCKII; Gap Junctions; signalering; segmentering;

    Sammanfattning : Calcium ions are one of the most versatile signalling molecules. They are essential tothe proper functioning of various cellular processes in many different types of cells.The calcium signals have been studied in the past using ratiometric dyes like Fura-2. LÄS MER

  4. 3. Investigation on Cell-Cell Junctions by Inhibition of Na,K-ATPase Activity

    Master-uppsats, KTH/Tillämpad fysik

    Författare :Caroline Boström; [2021]
    Nyckelord :NaK-ATPase; Ouabain; Cell junctions; ECadherin; Occludin; Claudin7; Western Blot; Fluorescent Imaging; Tight junctions; Adherens Junctions; Immunocytochemistry; Ouabain; NaK-ATPase; ECadherin; Occludin; Claudin7; Western Blot; Immunocytokemi; Cellkontakter; Fluorescernsmikroskopi;

    Sammanfattning : This thesis report investigates the effect on cellcell junction proteins when the Na,K-ATPase (NKA) is inhibited. The main goal is to develop an understanding of how the NKA activity regulates the cell junction proteins. The investigated proteins are the adherens junction protein ECadherin, and the tight junction proteins Occludin and Claudin7. LÄS MER

  5. 4. Ouabain Toxicity -Selectivity Towards Renal Cancer Cells

    Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Författare :Emma Magnusson; [2020]
    Nyckelord :Cardiotonic steroids; ouabain; Na K -ATPase; cytotoxicity; renal cancer; apoptosis; Kardiotoniska steroider; ouabain; Na K -ATPase; cytotoxicitet; njurcancer; apoptos;

    Sammanfattning : Ouabain and other cardiotonic steroids are known to inhibit Na + ,K + -ATPase (NKA), theion pump responsible for the ionic gradient across the plasma membrane. These steroidsdisplay a selective toxicity towards several tumour cells in comparison to primary humancells, however, the mechanism behind this is not yet understood. LÄS MER

  7. 5. Can Ouabain Protect Transplantation Kidneys fromApoptosis?- Construction of an Experimental Set-Up to StudyStorage-Induced Apoptosis -

    Master-uppsats, KTH/Tillämpad fysik

    Författare :Jana Hanke; [2014]
    Nyckelord :;

    Sammanfattning : .... LÄS MER