Sökning: "plasmid"

Visar resultat 1 - 5 av 93 uppsatser innehållade ordet plasmid.

  1. 1. Gene expression editing in myeloma cell lines using CRISPR/Cas9 technique

    Kandidat-uppsats, Uppsala universitet/Institutionen för medicinsk cellbiologi

    Författare :Wilma Wadman; [2023]
    Nyckelord :BMPR1A; BMPR2; bone morphogenetic protein; gene knockout; multiple myeloma;

    Sammanfattning : Multiple myeloma, or myeloma, is a bone marrow cancer which characterizes by uncontrolled proliferation of mutant plasma cells. It is a disease that claims many lives every year, mostly due to the absence of curative treatment. Finding a suitable treatment is therefor of great importance. LÄS MER

  3. 2. Characterization of encystation in Giardia intestinalis

    Master-uppsats, Uppsala universitet/Institutionen för cell- och molekylärbiologi; Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Johanna Schwarz; [2023]
    Nyckelord :Giardia intestinalis; parasite; encystation; transcription factors;

    Sammanfattning : Giardia intestinalis is a protozoan parasite causing the diarrheal disease called giardiasis that infects millions of people worldwide each year. The life cycle of Giardia intestinalis is characterized by two stages; the sturdy, infectious cyst and the vegetative, motile trophozoite. LÄS MER

  4. 3. Insulated Metabolic Exchange for Sustainable Agriculture and Biotechnology

    Master-uppsats, KTH/Industriell bioteknologi

    Författare :Ellen Engquist; [2023]
    Nyckelord :Biofertilisers; nitrogen; cyanobacteria; E. coli; allantoin; Biogödsel; kväve; cyanobakteria; E. coli; allantoin;

    Sammanfattning : Mellan 1960 och idag har världspopulationen dubblerats medan matproduktionen tredubblats. Det har möjliggjorts av kemiskt framställda gödsel som introducerades på mitten av 1900-talet. Sedan 1965 har användandet av kvävegödsel ökat sexdubblet. LÄS MER

  5. 4. Navigating Large-Scale Plasmid DNA Purification : A Recommendation of Current and Future Downstream Purification Solutions

    Kandidat-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Matilda Eriksson; Alva Wells; Maria Frey; Lisa Johansson; Gabriel Pettersson; David Sjöberg; [2023]
    Nyckelord :Plasmid purification; pDNA; large-scale chromatography;

    Sammanfattning : Previous small-scale methods for plasmid DNA (pDNA) purification fail to meet theindustry’s demand for sufficient quantities. Greater volumes of bacterial lysates are a consequence of larger volumetric fermentations and traditional large-scale down-stream purification processes have some disadvantages and limitations. LÄS MER

  7. 5. Recombinant expression and purification of sugar beet Phytoglobins in Escherichia Coli BL21 (DE3) including metabolic engineering of the heme biosynthesis

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Författare :Pennie Lindblom; Olivia Geel; [2023]
    Nyckelord :Phytoglobin; Heme; δ-ALA; qPCR; SDS-PAGE; Bacterial cultivation; Escherichia coli; BvPgb1.2; ÄKTA; Applied biochemistry; Biology and Life Sciences;

    Sammanfattning : The protein hemoglobin (Hb) is vital for us humans to ensure enough oxygen replacement in the body. Hb-resembling proteins also exist in plants, these are called phytoglobins (Pgbs). Pgbs are very interesting since they can play a considerable role in the development of blood substitutes and iron supplements for human use. LÄS MER