Sökning: "polyacrylamide gel electrophoresis"

Visar resultat 1 - 5 av 6 uppsatser innehållade orden polyacrylamide gel electrophoresis.

  1. 1. PREPARATION AND CHARACTERIZATION OF BOVINE MEAT AND MILK FACTOR VESICLES/PARTICLES & IDENTIFICATION OF MSBI1 REP INTERACTING PROTEINS

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Farhana Manzoor; [2020]
    Nyckelord :Bovine meat and milk factors; multiple sclerosis; immunoprecipitation co-immunoprecipitation; protein dimers; mass spectrometry; immunodetection; cesium chloride density gradient centrifugation; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; vesicle; particle; interaction partner; pull-down assay.;

    Sammanfattning : Introduction: Bovine meat and milk factors (BMMF) frequently isolated from bovine meat and milk, are discussed as putative causative agents for breast, colon and possibly other cancers. The hypothesis was formulated based on earlier correlative studies indicating that BMMFs could indirectly trigger inflammation, accumulate mutational events via reactive oxygen and nitrogen species production which eventually provokes tumor formation. LÄS MER

  3. 2. Implementation of the mille-feuille nanofilter paper in the virus removal filtration of IgY purified from chicken egg yolk

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Michelle Dahlman; [2020]
    Nyckelord :;

    Sammanfattning : The purpose of this thesis was to test the mille-feuille nanofilter paper for a virus removal filtration from a potential protein-based nutraceutical. Therefore, chicken IgY was purified from egg yolk, with PEG precipitation for crude fractionation followed by thiophilic adsorption chromatography. LÄS MER

  4. 3. Understanding the Involvement of Leukocyte Cell-derived Chemotaxin 2 (LECT2) in Amyloidosis

    Master-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

    Författare :Lisa-Marie Erlandsson; [2019]
    Nyckelord :LECT2 amyloidosis; disulfide bonds; metal-binding; protein expression; refolding; purification; fluorimetry; CD; aggregation;

    Sammanfattning : Leukocyte cell-derived chemotaxin 2 (LECT2) is a zinc-binding multi-functional protein comprising three disulfide bonds, that is involved in multiple disorders of worldwide concern. Recently LECT2 was found to be involved in amyloidosis (ALECT2) and is believed to be the third most common form of systemic amyloidosis. LÄS MER

  5. 4. A metaproteomics-based method for environmental assessment : A pilot study

    Master-uppsats, Kemi; Tekniska högskolan

    Författare :Henric Fröberg; [2013]
    Nyckelord :environmental samples; metaproteomics; liquid chromatography; polyacrylamide gel electrophoresis; mass spectrometry;

    Sammanfattning : Metaproteomics, as a proteomic approach to analyse environmental samples, is a new and expanding field of research. The field promises new ways of determining the status of the organisms present in a sample, and could provide additional information compared to metagenomics. LÄS MER

  7. 5. Characterization of antibodies against mustard and development of immunological methods for the detection and quantification of mustard in foods

    Kandidat-uppsats, Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Johanna Almgren; [2007]
    Nyckelord :Mustard allergen; rocket-immunoelectrophoresis; ELISA; immunoblotting; immunodiffusion.;

    Sammanfattning : ABSTRACT Allergy to mustard has been reported for many years, in some cases as severe anaphylactic reactions. Recent studies imply that this allergy is increasing. Three major allergens have been isolated and characterised; Sin a 1 and Sin a 2 in yellow mustard (Sinapis alba), and Bra j 1 in oriental mustard (Brassica juncea). LÄS MER