Sökning: "protein refolding"

Visar resultat 1 - 5 av 11 uppsatser innehållade orden protein refolding.

  1. 1. Effects of mixed surfactant system on chemical and physical stability of human growth hormone

    Master-uppsats, Lunds universitet/Livsmedelsteknik och nutrition (master)

    Författare :Karnsinee Viriyayudhakorn; [2023]
    Nyckelord :human growth hormone; surfactant; refolding; protein stability; physical degradation; chemical degradation; pharmaceutical technology; Technology and Engineering;

    Sammanfattning : In protein formulation, addition of surfactants is commonly executed to enhance product stability and shelf-life. The reason is that surfactants can protect proteins against surface-induced aggregation. However, the interaction between surfactant and protein depends on the type of surfactant used. LÄS MER

  3. 2. Production and analysis of novel disulfide variants of Subtilisin Carlsberg

    Master-uppsats, Linköpings universitet/Kemi

    Författare :Anton Elfstrand; [2023]
    Nyckelord :Subtilisin Carlsberg; Disulfide; Protein Engineering; Kinetic Stability;

    Sammanfattning : Protein engineering has been used to alter the stability of proteins for several decades withmuch success, one approach being to introduce two cysteine residues that together form adisulfide bridge. The disulfide bridge can increase the Gibbs free energy of the transitionstate, thus increasing energy difference between the folded state and the unfolding transitionstate, leading to increased kinetic stability of the protein. LÄS MER

  4. 3. Structural and Functional Studies of Giant Proteins in Lactobacillus kunkeei

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Strukturbiologi

    Författare :Josefin Ågren; [2019]
    Nyckelord :structural biology; protein expression; Lactobacillus kunkeei; L. kunkeei; small angle X-ray scattering; SAXS; circular dichroism; CD; thermofluor; nano differential scanning fluorimetry; nanoDSF; honey bee; honey crop; extracellular protein;

    Sammanfattning : Lactobacillus kunkeei is one of the most abundant bacteria within the honey crop of the honey bee. Genome sequencing of L. kunkeei isolated from honey bees all over the world showed several genes unique for L. kunkeei. LÄS MER

  5. 4. Purification and refolding of a novel dipeptidyl peptidase III

    Kandidat-uppsats, Linköpings universitet/Kemi

    Författare :Lennie Jansson; [2019]
    Nyckelord :dipeptidyl peptidase III; DPP III; purification; refolding; protein; Arg-Arg-2NA;

    Sammanfattning : There is a continuous search for novel enzymes to complement the abilities of today’s commercially available enzyme and find tailor-fit alternatives to suit the diverse array of bio-based industries. One application could be to increase biogas yield by finding substrate degrading proteases that can be added to the anaerobic digestion process and survive degradation themselves. LÄS MER

  7. 5. Understanding the Involvement of Leukocyte Cell-derived Chemotaxin 2 (LECT2) in Amyloidosis

    Master-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

    Författare :Lisa-Marie Erlandsson; [2019]
    Nyckelord :LECT2 amyloidosis; disulfide bonds; metal-binding; protein expression; refolding; purification; fluorimetry; CD; aggregation;

    Sammanfattning : Leukocyte cell-derived chemotaxin 2 (LECT2) is a zinc-binding multi-functional protein comprising three disulfide bonds, that is involved in multiple disorders of worldwide concern. Recently LECT2 was found to be involved in amyloidosis (ALECT2) and is believed to be the third most common form of systemic amyloidosis. LÄS MER