Sökning: "proximity ligation assay"

Visar resultat 1 - 5 av 11 uppsatser innehållade orden proximity ligation assay.

  1. 1. The role of Platelet-derived growth factor receptor β-signaling in non-small cell lung cancer

    Kandidat-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning; Uppsala universitet/Institutionen för immunologi, genetik och patologi

    Författare :Louise Hellberg; [2022]
    Nyckelord :pathology; lung cancer; PLA; PDGFR;

    Sammanfattning : A high expression of stromal PDGFRβ is known to be a poor prognosis marker in several solid tumor types. However, the role of stromal PDGFRβ for non-small cell lung cancer (NSCLC) patients remains unclear. LÄS MER

  3. 2. Validation and optimization of multiplexInSitu PLA for signalling pathway analysis

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Tanay Kumar Sinha; [2021]
    Nyckelord :Chronic myeloid leukemia Tyrosine kinase inhibitors Proximity ligation assay Cross-reactivity Padlock Template;

    Sammanfattning : With the advent of Tyrosine kinase inhibitors (TKI) as a therapy for Chronic myeloid Leukemia (CML), the patients now enjoy a life expectancy close to that of the general population. But some patients do get unresponsive to the TKI treatment over time due to several mutations in the kinase domain of the BCR-ABL fusion protein, which further leads to activation of multiple signaling cascades within the leukemic cell, helping it survive and proliferate. LÄS MER

  4. 3. Assay development for in situ detection of autophagy-related protein-protein interactions for characterization of colorectal cancer

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :M. Karoliina Hirvonen; [2015]
    Nyckelord :Autophagy; In situ Proximity Ligation Assay; Colorectal cancer;

    Sammanfattning : Every year, more than a million people are diagnosed with colorectal cancer (CRC) that develops in the large intestine. It is one of the most studied cancers in the world but still more knowledge about how this cancer develops and acts is needed in order to use more effective ways to treat CRC. LÄS MER

  5. 4. Optimization of the multiplexed Proximity Ligation Assay for detection of blood-based biomarkers

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Martin Lundberg; [2014]
    Nyckelord :PLA; colorectal cancer; biomarker; multiplex qPCR; Olink;

    Sammanfattning : The Proximity Ligation Assay (PLA) is a relatively new method which utilizes the strength of both immunoassays and DNA detection. PLA has the capacity of high multiplexing due to the high specificity achieved with both dual protein-binding and dual primer binding during detection with Real-Time PCR. LÄS MER

  7. 5. Detection of IGF-1 and phosphorylated site in PDGFr using proximity ligation assay

    Magister-uppsats, SLU/Dept. of Microbiology

    Författare :Raghavendra Magalam ; [2014]
    Nyckelord :PLA; IGF-1; PDGFβr; IgG; recombinant PDGFr; VEGF; solid phase; solution phase;

    Sammanfattning : Proximity ligation assay (PLA) is a novel affinity based method used to detect proteins, protein-protein interactions and post-translational modifications. The detection of target molecules is achieved with the help of oligonucleotide labeled antibodies. LÄS MER