Sökning: "qPCR"

Visar resultat 1 - 5 av 105 uppsatser innehållade ordet qPCR.

  1. 1. Development of a novel quantitative PCR analysis method for HIV-1

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Teoretisk kemi

    Författare :Anna-Malin Hallberg; [2021]
    Nyckelord :HIV; PCR; primers; probes; virus; detection; blood plasma; pathogen; biological pharmaceutical; protein-based pharmaceutical; genotype; subtype; RT qPCR; extraction; TaqMa; RNA virus; cross-dimer; applied biochemistry; tillämpad biokemi; Biology and Life Sciences; Technology and Engineering; Chemistry;

    Sammanfattning : Biological pharmaceuticals are the answer to many severe diseases of today’s society. Some of these are protein-based drugs and can be produced from human blood plasma. In order to guarantee the safety of patients, absence of any pathogens needs to be ensured. LÄS MER

  2. 2. Mastocytom hos hund : analys av serglycinuttryck och proteasaktivitet i serum

    Uppsats för yrkesexamina på avancerad nivå, SLU/Dept. of Biomedical Sciences and Veterinary Public Health

    Författare :Elvira Palmborg; [2020]
    Nyckelord :mastocytom; cancer; serglycin; proteaser; tryptas; kymas; kallikrein; plasmin; trombin; substrat-assay; ELISA; peptidprofilering; MALDI-TOF; qPCR; biomarkör;

    Sammanfattning : .... LÄS MER

  3. 3. Assessment of methods for microRNA isolation, microRNA amplification, and development of a normalization strategy for sepsis biomarker research

    Master-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Johan Nordén; [2020]
    Nyckelord :;

    Sammanfattning : Sepsis, defined by organ dysfunction caused by an adverse immune response of the host to an infection, comes with considerable cost in human lives and as a substantial burden financially. Significant upgrades have been made over the past two decades when diagnosing and treating sepsis but still with room for improvements. LÄS MER

  4. 4. Development and validation of a novel quantitative PCR analysis method for HIV-1

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Teoretisk kemi

    Författare :Josefine Sundgren; [2020]
    Nyckelord :HIV-1; qPCR; Octapharma; Taqman; applied biochemistry; tillämpad biokemi; Technology and Engineering;

    Sammanfattning : The major problem when developing primers and probe sets for detection of HIV-1 is its high genetic variability and strong ability to mutate. HIV-1 is divided into four groups, M, N, O, P based on genetic similarities, and there are more than 70 different circulating recombinant forms. LÄS MER

  5. 5. Avloppsslam på åkermark : Analys av långsiktiga effekter på mikrobsamhället och kväveomvandlande mikroorganismer

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Elin Malmström; [2020]
    Nyckelord :avloppsslam; nitrifikation; denitrifikation; mikrobsamhälle;

    Sammanfattning : One major environmental challenge is to manage agricultural soil in asustainable way. Since nutrients in the soil are taken away every harvest, there is a need to recycle nutrients back to the soil. One solution is to use sewage sludge as a fertilizer. However, sludge can also contain substances that could potentially be harmful to crops or humans. LÄS MER