Sökning: "recombinant food proteins"

Visar resultat 1 - 5 av 6 uppsatser innehållade orden recombinant food proteins.

  1. 1. Characterization of ulvan polysaccharide degrading enzymes from Wenyingzhuangia fucanilytica

    Master-uppsats, Lunds universitet/Bioteknik; Lunds universitet/Bioteknik (master)

    Författare :Erik Apelqvist; [2022]
    Nyckelord :Enzyme; Enzymatic characterization; Recombinant protein producaiton; Protien purification; E. coli; W. fucanilytica; Transformation; Overexpression; Seaweed; Ulvan; Polysaccharides; biotechnology; Biology and Life Sciences;

    Sammanfattning : The aim of this thesis was to fully characterize the enzyme WFp-105, including bioinformatic analysis, structural modeling, full activity and stability characterization and product determination. As well as for the enzyme WFp-37; optimize protein production in regard to expression strains and cultivation temperature, analyzing the genomic context as well as performing an initial activity detection. LÄS MER

  3. 2. Evaluation of strategies for construction of protease libraries in yeast.

    Master-uppsats, Lunds universitet/Teknisk mikrobiologi; Lunds universitet/Bioteknik

    Författare :Sidharth Jaya Sankar; [2022]
    Nyckelord :Yeast; GFP-fusion technology; protease; applied microbiology; Technology and Engineering;

    Sammanfattning : Proteases are a group of enzymes that are involved in breaking down larger proteins into smaller polypeptides or single amino acids, and they are used in various applications. In this project, the potential of budding yeast Saccharomyces cerevisiae as host for the construction of protease libraries is evaluated. LÄS MER

  4. 3. Study of fibrillation processes of amyloid-like β-lactoglobulin protein

    Master-uppsats, KTH/Kemi

    Författare :Jose Nixon; [2021]
    Nyckelord :bLG; Thioflavin-T Fluroscence; Fibrillation; bLG; Thioflavin-T fluroscens; fibrillering;

    Sammanfattning : Bovint β-laktoglobulinprotein (bLG) är ett litet globulärt protein med 162 aminosyrarester, som vanligtvis finns i mjölkvassle. Under sura förhällanden dissocierar dessa dimera proteiner och bildar amyloidliknande fibriller. LÄS MER

  5. 4. Recombinant Food Proteins Expressed in E. coli as Complement to Plant Proteins in the Protein Shift

    Master-uppsats, Lunds universitet/Livsmedelsteknik och nutrition (master)

    Författare :Anthon Izad Khast Wellerfeld; [2021]
    Nyckelord :recombinant food proteins; protein shift; protein functionality; plant-based; meat analogs; dairy; food technology; Technology and Engineering;

    Sammanfattning : Increased awareness regarding nutrition, environmental sustainability, and animal welfare has sparked a trend to shift away from animal proteins. The phenomenon is referred to as the protein shift and has resulted in a higher demand for alternative protein sources. LÄS MER

  7. 5. Optimizing signal peptides for expression of recombinant antibodies in HEK293 cells

    Master-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

    Författare :Gustav Myhrinder; [2020]
    Nyckelord :Signal peptide; antibody; mammalian cell; HEK293; secretory pathway;

    Sammanfattning : Monoclonal antibodies are well-established as a therapeutic in the biopharmaceutical market, targeting a variety of diseases and with 79 approved products by the United States Food and Drug Administration in December 2019. Therapeutic monoclonal antibodies are commonly produced as recombinant proteins in mammalian cell lines, due to their capacity of post-translational modifications, most notably glycosylation. LÄS MER