Sökning: "single-particle imaging"

Visar resultat 1 - 5 av 7 uppsatser innehållade orden single-particle imaging.

  1. 1. Engineered imaging scaffolds for cryo-EM of small proteins of interest

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Oscar Friberg; [2022]
    Nyckelord :Protein structure determination; single-particle cryo-EM; imaging scaffold; size limitation problem; affibody; YgjG.;

    Sammanfattning : Strukturbestämning av proteiner är viktigt för att kunna förstå deras funktion och en snabbt utvecklande metod inom fältet är kryoelektronmikroskopi. Storleksbegränsningar förhindrar en bredare applikation av metoden eftersom små proteiner har för låg signal i förhållande till bakgrund för att kunna visualiseras som enstaka partiklar i elektronmiksoskopibilder. LÄS MER

  3. 2. Diffusion and Camera-Noise Modelling for Analysis of Single-Particle Tracking Movies

    Master-uppsats, Lunds universitet/Beräkningsbiologi och biologisk fysik - Genomgår omorganisation

    Författare :Erik Clarkson; [2022]
    Nyckelord :reaction-diffusion; camera-noise modelling; single-particle tracking; Gillespie simulations; Physics and Astronomy;

    Sammanfattning : Interactions between T-cells and other body cells is an essential part of the immune system. It involves the binding between surface receptors of the two cells. Specifically, T-cell receptors (TCRs) bind onto pMHC (peptide-loaded major histocompatibility complex) molecules on the partnering cell surface. LÄS MER

  4. 3. Polarizability and Orientation Dynamics of Small Proteins

    Master-uppsats, Uppsala universitet/Materialteori

    Författare :Ebba Koerfer; [2022]
    Nyckelord :Protein orientation; single-particle imaging; polarizability; orientation dynamics; rigid rotor model;

    Sammanfattning : Proteins often carry an intrinsic electric dipole moment, which can interact with external electric fields and cause protein motion. Previous research has found that the orientation of small proteins in gas phase can be controlled in a static electric field. LÄS MER

  5. 4. The effect of water on the orientation of a protein in an electric field

    Master-uppsats, Uppsala universitet/Institutionen för fysik och astronomi

    Författare :Gideon Elfrink; [2022]
    Nyckelord :Structure determination; Gromacs; tutorial; Molecular Dynamics; protein; ubiquitin;

    Sammanfattning : Structure determination of proteins is vital for the understanding of their function. It often relies on techniques that use intense X-ray pulses to create diffraction patterns of protein crystals, which then contain information on the three-dimensional structure of the crystallised protein. LÄS MER

  7. 5. Sustaining Orientation of Ubiquitin for Single Particle Imaging Using Electric Fields

    Kandidat-uppsats, Uppsala universitet/Kemisk och biomolekylär fysik; Uppsala universitet/Energimaterialens fysik

    Författare :Adi Bijedic; [2021]
    Nyckelord :;

    Sammanfattning : Single-particle imaging, or SPI, is a method used to obtain the three-dimensional structure of particles. Repeatedly aiming X-rays at samples of a particle produces diffraction patterns, which are combined to a best-fit three-dimensional model of the particle. SPI of proteins can be improved by orienting the protein before imaging. LÄS MER