Sökning: "structural genes"

Visar resultat 1 - 5 av 25 uppsatser innehållade orden structural genes.

1. 1. Approaches to Structural Characterization of a Heteromeric GABA(A)R

   Master-uppsats, KTH/Tillämpad fysik

   Författare :Alexander Stevens; [2023]
   Nyckelord :Applied Physics; Biophysics; Molecular Biophysics; GABA A R; LGIC; pLGIC; Tillämpad Fysik; Biofysik; Molekylär Biofysik; GABA A R; LGIC; pLGIC;

   Sammanfattning : Structural biology has become an important part of researching various diseases and drug development. In this thesis, I provide details on how I worked with approaches to structural characterization of a heteromeric GABA(A)R. LÄS MER

3. 2. Investigations of miR-34a structure and dynamics by SHAPE MaP and optimal time between transfection and modification by qPCR

   Master-uppsats, KTH/Proteinvetenskap

   Författare :Ellinor Lindén; [2023]
   Nyckelord :SHAPE MaP; qPCR; miRNA; HNF4α; mRNA-34a; SHAPE MaP; qPCR; miRNA; HNF4α; mRNA-34a;

   Sammanfattning : microRNA (miRNA) är korta icke-kodande RNA sekvenser som har fått ökande uppmärksamhet då de kan binda till mRNA och styra vårt genuttryck. Omkring trettio procent av våra proteinkodande gener styrs av miRNA. miRNA binder till mRNA vilket hindrar dess translation eller leder till nedbrytning av mRNA. LÄS MER

4. 3. Characterization of ulvan polysaccharide degrading enzymes from Wenyingzhuangia fucanilytica

   Master-uppsats, Lunds universitet/Bioteknik; Lunds universitet/Bioteknik (master)

   Författare :Erik Apelqvist; [2022]
   Nyckelord :Enzyme; Enzymatic characterization; Recombinant protein producaiton; Protien purification; E. coli; W. fucanilytica; Transformation; Overexpression; Seaweed; Ulvan; Polysaccharides; biotechnology; Biology and Life Sciences;

   Sammanfattning : The aim of this thesis was to fully characterize the enzyme WFp-105, including bioinformatic analysis, structural modeling, full activity and stability characterization and product determination. As well as for the enzyme WFp-37; optimize protein production in regard to expression strains and cultivation temperature, analyzing the genomic context as well as performing an initial activity detection. LÄS MER

5. 4. Investigation of Chromatin Organization and mRNA Expression in Drug Treated Human Erythroleukemia Cells

   Master-uppsats, KTH/Proteinvetenskap

   Författare :Anam Minhas; [2022]
   Nyckelord :Erythroleukemia cells; mRNA-seq; ATAC-seq; Doxorubicin; Vinblastine; Erytroleukemiceller; mRNA-expression; kromatin; Doxorubicin; Vinblastine;

   Sammanfattning : Syftet med detta projekt var att undersöka hur vanligt använda cancerläkemedel påverkar mRNA-uttryck och kromatinorganisation i humana erytroleukemiceller. Som modell användes K562-celler från en patient i blastocystkris (2), för att utvärdera leukemicellernas svar på cancerläkemedel vinblastin och doxorubicin. LÄS MER

7. 5. Design, expression and purification of virus-like particles derived from metagenomic studies : Virus-like Particles (VLP) of novel Partitiviridae species, Hubei.PLV 11, and novel Soutern pygmy squid flavilike virus were designed, expressed using the bac-to-bac expression system and then pruified using various methods

   Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

   Författare :Diyar Ayranci; [2021]
   Nyckelord :Virus; virus-like particles; VLP. Western Blot; SDS-PAGE; Chromatography; partitivrus; partitiviridae; flaviviridae; flavivirus; Hubei partitilike virus; Southern Pygmy Squid flavilike virus; protein purification; protein characterization; PCR; Cloning; metagenomic; strucutral biology; cryo-em; bac-to-bac expression system; vaccines; arbovirus; arthropod-borne virus;

   Sammanfattning : Viruses are entities which are made of a few genes and are reliant on obligate parasitism to propagate. Due to the obligate connection to their hosts, virus evolution is constrained to the type of host. Viruses however do transmit to evolutionary distinct hosts; in these cases, the phylogenetic relationship of the hosts usually are close. LÄS MER