Sökning: "substrate specificity"

Visar resultat 1 - 5 av 14 uppsatser innehållade orden substrate specificity.

  1. 1. In silico and in vitro determination of substrate specificity for Breast Cancer Resistance Protein (BCRP) transporter at the blood-brain barrier

    Master-uppsats, Uppsala universitet/Institutionen för farmaci

    Författare :Fen Wang; [2021]
    Nyckelord :BCRP; substrate specificity; BBB; ATP-binding cassette ABC transporter; Drug transport;

    Sammanfattning : Background The Breast Cancer Resistance Protein (BCRP) drug transporter is important for drug disposition and plays a critical role in regulating drug entry into the brain. Its substrate spectrum overlaps with substrates of Multi Drug Resistance Protein 1 (MDR1, P-gp), which influences and complicates the interpretation of data on drug distribution into tissues (e. LÄS MER

  2. 2. Exploring an alternative metabolic pathway for production of adipic acid - a study on homocitrate synthase

    Master-uppsats, Lunds universitet/Examensarbeten i molekylärbiologi

    Författare :Damaride Stallone; [2019]
    Nyckelord :Biology and Life Sciences;

    Sammanfattning : Adipic acid is considered as the most valuable dicarboxylic acid with industrial use; however, its production is heavily petroleum-based. This non-natural metabolite can be produced de novo through different metabolic pathways, one of which is the alpha-ketoacid elongation pathway. LÄS MER

  3. 3. Determination of specificity and affinity of the Lactose permease (LacY) protein of Escherichia coli through application of molecular dynamics simulation

    Master-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Stuart Lutimba; [2018]
    Nyckelord :;

    Sammanfattning : Proteins are essential in all living organisms. They are involved in various critical activities and are also structural components of cells and tissues. LÄS MER

  4. 4. Enzyme engineering of a haloacid dehalogenase-like phosphatase from Thermotoga neopolitana for optimization of substrate specificity

    Master-uppsats, Lunds universitet/Bioteknik (master)

    Författare :Samuel Sutiono; [2016]
    Nyckelord :Catalytic efficiency; Haloacid dehalogenase-like hydrolase; Enzyme engineering; Thermal stability; Inhibition; biotechnology; bioteknik; Technology and Engineering;

    Sammanfattning : Haloacid dehalogenase (HAD)-like hydrolases represent a vast superfamily characterised by their ability to form covalent enzyme-substrate intermediates facilitating the cleavage of C-Cl, C-P, or CO-P bonds. Within this work one member of this superfamily, HAD from Thermotoga neopolitana (HADTn) exhibiting a phosphatase (P-O bond cleavage) like activity was investigated in the context of altering the substrate specificity. LÄS MER

  5. 5. Cloning, Expression and Purification of a Mutant Tomato Thymidine Kinase 1 for Crystallography

    Master-uppsats, Lunds universitet/Tillämpad biokemi

    Författare :Mohamad Nesrini; [2015]
    Nyckelord :protein expression; protein engineering; Azidothymidine; thymidine kinase; deoxyribonucleoside kinases; nucleosides; nucleoside analog; suicide gene therapy; tillämpad biokemi; applied biochemistry; BEVS.; Technology and Engineering; Chemistry;

    Sammanfattning : Thymidine kinase 1 from tomato (ToTK1) is a newly discovered deoxyribonucleoside kinase (dNK) that has been subject to study due to its in vitro and in vivo properties making it an interesting suicide gene candidate for the treatment of brain tumors. The ToTK1 phosphorylates the nucleoside analogue 3-azido-2,3-dideoxythymidine (azidothymidine, AZT) equally well as its natural substrate thymidine (dThd). LÄS MER