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  1. 1. Recombinant expression and purification of sugar beet Phytoglobins in Escherichia Coli BL21 (DE3) including metabolic engineering of the heme biosynthesis

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Författare :Pennie Lindblom; Olivia Geel; [2023]
    Nyckelord :Phytoglobin; Heme; δ-ALA; qPCR; SDS-PAGE; Bacterial cultivation; Escherichia coli; BvPgb1.2; ÄKTA; Applied biochemistry; Biology and Life Sciences;

    Sammanfattning : The protein hemoglobin (Hb) is vital for us humans to ensure enough oxygen replacement in the body. Hb-resembling proteins also exist in plants, these are called phytoglobins (Pgbs). Pgbs are very interesting since they can play a considerable role in the development of blood substitutes and iron supplements for human use. LÄS MER

  3. 2. Characterization of potato protein and its calcium binding capacity

    Master-uppsats, Lunds universitet/Beräkningskemi; Lunds universitet/Tillämpad biokemi

    Författare :Signe König; Erika Thomson; [2023]
    Nyckelord :Patatin; Calcium; Food supplement; Calcium Assay; Alcalase; potato protein; Applied biochemistry; Biology and Life Sciences; Technology and Engineering;

    Sammanfattning : Calcium is essential for the human body as it has functions in the nervous system, bones and formation of teeth. Deficiency in this important nutrient can be detrimental to health and cause life-long issues. Calcium can be found in various foods, like dairy products, but certain plant-based alternatives to milk needs to be fortified with calcium. LÄS MER

  4. 3. Recombinant Expression of Novel Wild Type Oat Hemoglobins in Escherichia Coli

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Författare :Frank Rummukainen; [2022]
    Nyckelord :Recombinant technology; Protein expression; Hemoglobin; Phytoglobin; Escherichia coli; Oat; Avena sativa; Heme; Iron; Biosynthesis; Transcription; qPCR; SDS-PAGE; Fermentation; Recombination; Applied Biochemistry; Biology and Life Sciences;

    Sammanfattning : Hemoglobin is a protein transporting oxygen through our bloodstreams and is as such essential for aerobic life. Hemoglobins also exist in plants, with a wide variety of functions other than oxygen transport. LÄS MER

  5. 4. Molecular Characterization of High β-Glucan Lines in Oats

    Master-uppsats, Lunds universitet/Tillämpad biokemi

    Författare :Abhinav Majumdar; [2022]
    Nyckelord :Applied biochemistry; Oats; β-Glucan; Dietary fibres; Arbinoxylan; Biology and Life Sciences;

    Sammanfattning : Oats (Avena sativa) are part of the cereal whole grain family, and their production is on the rise for the past few years due to extensive research done on the health benefits it can provide to humans. ScanOats project in Sweden is one of the research groups across the world that has done extensive work on oats. LÄS MER

  7. 5. EXPRESSION OF SUGAR BEET HEMOGLOBIN IN PICHIA PASTORIS

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Författare :Johan Pettersson; [2022]
    Nyckelord :Pichia pastoris; expression; sugar beet; hemoglobin; km71; ppic9; ppic3.5; phil-s1; applied biochemistry; Biology and Life Sciences;

    Sammanfattning : People who don’t eat meat can suffer from iron deficiency due to the lower efficiency of absorption of non-heme iron compared to heme iron. The aim of this master thesis was to explore the possibilities of producing a plant-based alternative of heme-iron by evaluating the efficiency of expression of sugar beet hemoglobin in the methylotrophic yeast Pichia pastoris. LÄS MER