Sökning: "transcriptome"

Visar resultat 1 - 5 av 13 uppsatser innehållade ordet transcriptome.

  1. 1. Increased expression of therapeutic proteins by identification of 3'-UTRs from high expressing genes in CHO cells

    Master-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

    Författare :Alexander Westlund; [2019]
    Nyckelord :Mammalian cell; CHO; E. coli; protein expression; plasmid; transformation; Fc-fusion protein; flow cytometry; FACS; transfection; mRNA; UTR; gene engineering;

    Sammanfattning : Therapeutic proteins, a.k.a. biopharmaceuticals, are most commonly produced in expression systems derived from Chinese Hamstery Ovary (CHO) cells, thanks to great capacity of post-translational modifications like secretation, folding and glycosylation. LÄS MER

  2. 2. Mimicking embryonic interactions with engineered cell-culturing techniques

    H-uppsats, Chalmers tekniska högskola/Institutionen för biologi och bioteknik

    Författare :Jakob Obermüller; [2017]
    Nyckelord :compaction; filopodia; embryo; fibroblasts.;

    Sammanfattning : Compaction of the mouse embryo is triggered by the formation of filopodia by some of the blastomeres. These finger-like processes extend onto neighboring cells, provid-ing mechanichal tension and possibly sending a signal that mediates compaction [1]. LÄS MER

  3. 3. Increasing bioinformatics in third world countries : Studies of S.digitata and P.Polymyxa to further bioinformatics in east Africa

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Sylvin Isak; [2016]
    Nyckelord :bioinformatics; pipeline; Setaria digitata; Paenibacillus polymyxa; Eleusine coracana; annotation; expression level; east Africa; eBioKit; MAKER; Cufflinks; third world country; genome; transcriptome;

    Sammanfattning : Despite an increase of biotechnical studies in third world countries, the bioinformatical side is largely lacking. In this paper we attempt to further the bioinformatical capabilities of east Af-rica. LÄS MER

  4. 4. Integrative analysis of age-related changes in the transcriptome of Caenorhabditis elegans

    Magister-uppsats, Högskolan i Skövde/Institutionen för biovetenskapHögskolan i Skövde/Forskningscentrum för Systembiologi

    Författare :Tsimafei Padvitski; [2015]
    Nyckelord :Aging; gene expression; RNA-seq; transcription regulation;

    Sammanfattning : Ageing is difficult to study because of the complexity and multi-factorial nature of traits that result from a combination of environmental, genetic, epigenetic and stochastic factors, each contributing to the overall phenotype. In light of this challenge, transcriptomic studies of aging organisms are of particular interest, since transcription is an intermediate step that links genotype and phenotype. LÄS MER

  5. 5. RNA-Seq: Yellow Nutsedge (Cyperus esculentus) transcriptome analysis of lipid-accumulating tubers from early to late developmental stages

    Master-uppsats, SLU/Dept. of Animal Breeding and Genetics

    Författare :Axel Thieffry; [2014]
    Nyckelord :Cyperus esculentus; transcriptome; tubers; RNA-seq; bioinformatics;

    Sammanfattning : Thanks to high amounts of starch and oil amassed in the parenchyma of its tubers, yellow nutsedge (Cyperus esculentus) stands as a unique plant species with regards to nutrient biosynthesis and accumulation in underground organs. In the last decades, understanding of enzymatic processes in lipid, starch and sugar pathways underwent great improvements. LÄS MER