Sökning: "ubiquitin"

Visar resultat 1 - 5 av 20 uppsatser innehållade ordet ubiquitin.

  1. 1. Imaging of the Cytosolic Antibody Receptor TRIM21

    Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Författare :Þórunn Stefánsdóttir; [2022]
    Nyckelord :TRIM21; ProteinStructure; Antibody; cryo-EM; Gel-Filtration SizeExclusion Chromatography ; Immunology; ProteinPurification; SDS-PAGE; TRIM21; Proteinstruktur; Antikroppar; cryo-EM; Gel-filtreringsKromatografi; Immunologi; ProteinRening; SDS-PAGE;

    Sammanfattning : TRIM21 is a cytosolic ubiquitin ligase and an antibody receptor that providesa last line of defense against invading pathogens. By utilizing the diversity ofantibody repertoire to identify pathogens, TRIM21 serves as a link betweenintrinsic cellular defense and adaptive immunity. LÄS MER

  3. 2. Polarizability and Orientation Dynamics of Small Proteins

    Master-uppsats, Uppsala universitet/Materialteori

    Författare :Ebba Koerfer; [2022]
    Nyckelord :Protein orientation; single-particle imaging; polarizability; orientation dynamics; rigid rotor model;

    Sammanfattning : Proteins often carry an intrinsic electric dipole moment, which can interact with external electric fields and cause protein motion. Previous research has found that the orientation of small proteins in gas phase can be controlled in a static electric field. LÄS MER

  4. 3. The effect of water on the orientation of a protein in an electric field

    Master-uppsats, Uppsala universitet/Institutionen för fysik och astronomi

    Författare :Gideon Elfrink; [2022]
    Nyckelord :Structure determination; Gromacs; tutorial; Molecular Dynamics; protein; ubiquitin;

    Sammanfattning : Structure determination of proteins is vital for the understanding of their function. It often relies on techniques that use intense X-ray pulses to create diffraction patterns of protein crystals, which then contain information on the three-dimensional structure of the crystallised protein. LÄS MER

  5. 4. Sustaining Orientation of Ubiquitin for Single Particle Imaging Using Electric Fields

    Kandidat-uppsats, Uppsala universitet/Kemisk och biomolekylär fysik; Uppsala universitet/Energimaterialens fysik

    Författare :Adi Bijedic; [2021]
    Nyckelord :;

    Sammanfattning : Single-particle imaging, or SPI, is a method used to obtain the three-dimensional structure of particles. Repeatedly aiming X-rays at samples of a particle produces diffraction patterns, which are combined to a best-fit three-dimensional model of the particle. SPI of proteins can be improved by orienting the protein before imaging. LÄS MER

  7. 5. Development of biophysical methods for characterization of PROTACs

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Biokemi

    Författare :Westlin Oskar; [2021]
    Nyckelord :;

    Sammanfattning : Targeting protein with the aim of ubiquitin-labelling for degradation is a new and upand coming field of drug discovery. Proteolysis targeting chimeras (PROTACs) areheterobifunctional linker molecules that connects the target molecule to an E3ubiquitin ligase, subsequent ubiquitination of the target protein initiates the proteindegradation by the proteosome system. LÄS MER