Daggmaskmetabolism: En potentiell bioindikator vid sanering av petroleumförorenad mark - En litteraturstudie

Sammanfattning: Remediating petroleum contaminated soil is a big task. Ex situ methods involve digging up, transporting, remediating and returning contaminated soil which is expensive and environmentally hazardous. Therefore in situ methods of soil remediation are being developed. However, these methods require some form of validation. Due to soil being a complex below ground matrix with large biological and physical variation this isn’t a simple matter. This literature review examines the feasibility of a novel method for validating in situ bioremediation. The proposed method is to extract inoculated earthworms from the soil several times during bioremediation and examine their metabolomics using 1H NMR spectroscopy. The resulting metabolomics profiles are then analyzed as a time series using PCA to discern changes in response to petroleum. This proposed method is based on research which is still in its infancy and thus some questions remain to be answered before application is possible. The two main questions that remain to be answered are how clearly the metabolomics response to petroleum can be discerned from background variation and to what extent earthworms avoid petroleum in the field. However, assuming the questions raised are answered favorably, the proposed method can be of interest under the right circumstances due to its potential to measure actual field effects on an ecosystem engineer with high sensitivity.