Gene expression analysis of fibroblast growth factor in a rat model of human endometrial cancer

Sammanfattning: The fibroblast growth factor receptor (FGF/FGFR) signaling has a significant role in normal organ development, like vascular and skeletal development. The dysregulation of the fibroblast growth receptor signaling occursdue to genetic modification or over expression of the receptor. This has been observed in different carcinomas [34,35].The endometrial carcinoma is the most common gynecologic malignancy in western counties and fourth most common cancer among women worldwide.Type I endometrial carcinomas constitute approximately 70 to 80% of all endometrium cancer.Itfollows estrogens related pathways in carcinogenesis. The BDII rat model is an ideal model for hormonal carcinogenesis because 90% of the female virgin spontaneously develop type I hormone independent endometrial adenocarcinomas within two years of age. Fibroblast growth factor (FGF) ligands via FGFR combined with heparan sulfate proteoglycans (HPSG) in extracellular matrix with the help of proteases and participate in the signal transmission of various functions such as cell proliferation, cell survival, cell motility. FGF signaling pathway is found to be aberrantly expressed in many of the cancers such as prostate and breast carcinomas. The main aim of the study was to investigate the differential expression of FGF gene in the malignant and nonmalignant cell lines of BDII rat endometrial strains along with human embryonic kidney cell& Ishikawa cell lines. [1, 2, 34, 35, 36,]. Real-time qPCR was used for analyzing relative expression of FGF gene between malignant and non-malignant cell lines in this study. The result showed a higher trend of the FGF gene in the non-malignant cell lines, compared to the expression in the malignant cell lines. It can be conspicuously seen that the FGF gene has shown higher trend in the Ishikawa cell line but cannot conclude about significantly over expression of FGF gene in this study due to very low number of samples. Moreover, this study should confirm by using additional techniques such as Western blot, Immunofluorescence to check the expression of FGF at protein level and cellular level.