THE TRANSCRIPTIONAL LANDSCAPE OF B CELLS UPON MUCOSAL HEALING IN EXPERIMENTAL COLITIS : A SINGLE CELL TRANSCRIPTOMICS ANALYSIS

Sammanfattning: Upon damage of the intestinal barrier, a complex interaction between the immune system and the epithelium is required to promote mucosal healing. However, which cell type and how the immune system orchestrates changes in the tissue transcriptomic landscape to eventually modulate mucosal healing is yet to be investigated. Here, using scRNA-seq, I identified a particular cluster of cells that increased the most during mucosal healing and is characterized by the expression of serine proteases inhibitors and for having a higher JAK/STAT activation signature. Further characterization of the stromal cell and intestinal epithelial network cor- roborate that B cells modulate their transcriptomic profiles related to tissue remodelling. All in all, this data suggests that B cells might accumulate in the colonic tissue during mucosal healing to influence the transcriptomic landscape associated with tissue remodeling.