Analysis of chlorophyll synthesis mutants xantha-m and xantha-j of Hordeum vulgare L.

Detta är en Kandidat-uppsats från Lunds universitet/Examensarbeten i molekylärbiologi

Författare: Fredrik Klinghammer; [2020]

Nyckelord: Biology and Life Sciences;

Sammanfattning: The xantha phenotype of barley is observable as a yellow plant and caused by an inability to correctly synthesize chlorophyll. Chlorophyll is a tetrapyrrolic pigment containing magnesium, and is integral for photosynthesis in plants. The phenotype can be caused by a number of mutations, and through methods like linkage mapping and sequencing it is possible to find the causative genotype. In this project xantha mutants xan-m and xan-j were investigated. The xan-m mutants were thought to possess mutations in the uncategorized gene HORVU3Hr1G050010 and xan-j a mutation in the chlorophyll synthase gene. The candidate gene of xan-m mutants is very large, and so only its transcribed mRNA was investigated through mRNA-selective RT-PCR and sequencing of the gene in eight fragments. A previously known mutation in the candidate gene of mutant xan-m.53 was in this project confirmed through sequencing. However, no other sequenced xan-m mutants possessed mutations in the candidate gene.This suggests that the candidate gene mutation is not the one causing the xantha phenotype. xan-j investigation through western blotting was during the project unsuccessful, due to ordered antibodies not being of sufficient concentration.

  HÄR KAN DU HÄMTA UPPSATSEN I FULLTEXT. (följ länken till nästa sida)