Forming the protein corona on nanoparticles

Sammanfattning: Nanomedicine is a growing field with great potential. When nanoparticles (NPs) come in contact with biological fluids a protein corona is formed on the surface. The protein corona has an effect on the pharmacokinetics and biological response. This might in turn lead to disadvantageous drug delivery and needs to be addressed when using NPs as drug delivery tools. How to build the protein corona and what effect different proteins have is a live research topic. In this study we investigate whether adding the same volume of polystyrene-COOH NPs, but in different aliquots, into a solution of immunoglobulin G (IgG) and other proteins has an effect of protein corona formation and NP aggregation. The aggregation formation is studied with the help of dynamic light scattering and absorbance measurements. We observed that that higher protein concentration did not lead to higher aggregation. The highest aggregation was found to form at a concentration of 0.1 mg/ml IgG. When the NPs were added in different aliquots the final aggregation was found to be larger compared to a single addition alone. Comments about weakness, improvements and potential use of the study are offered. An outlook on the future field is also briefly discussed.