Exponering av bisfenol A från fiskkonserver

Sammanfattning: Bisphenol A (BPA) is an endocrine disruptor which is very present in our surrounding environment. As a component mainly used in plastics and in the inner linings in cans, BPA can also be found in our bodies due to migration into food. Once the substance is reaching the components of the body it is biotransformed into a more water soluble molecule called monoglucoronide. Whether to measure the unconjugated BPA or the monoglucoronide in biological samples is, in the world of scientists, still debated. Many hold that only the unconjugated BPA is acting as a endocrine disruptor but contamination of BPA from materials in lab could obscure the true levels of exposure. One could try the best to avoid contamination or instead measure the levels of monoglucoronide. Both methods were conducted in this study in order to examine if there were any exposure of BPA from canned fish to humans. 21 volunteers donated urine and blood samples after having a lunch consisting of different fishes in cans. The samples were then analyzed for unconjugated BPA and monoglucoronide in urine. Solely unconjugated BPA was analyzed in blood serum by two different techniques - one with enzyme and one without. The analyses were performed by liquid chromatography-tandem mass spectrometry. The results revealed elevated levels of BPA in urine and in blood serum samples, with enzyme, several hours after lunch. Monoglucoronide in urine was partly detected when comparing with earlier analysis executed by the Occupational and Environmental Medicine at Lund University. This could be due to the present of other metabolites. Eating sardines or tuna gave a significant elevation of BPA in urine. Hence canned fish is a source of exposure of BPA to humans. This study demonstrates the ubiquitous nature of BPA in our daily life.