Sökning: "CHO-cell"

Visar resultat 1 - 5 av 7 uppsatser innehållade ordet CHO-cell.

  1. 1. OPTIMIZATION OF CLONE SELECTION PLATFORM FOR PRODUCTION OF RECOMBINANT PROTEINS

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Basel Yousef; [2024]
    Nyckelord :;

    Sammanfattning : Background  Biologics are type of proteins that can be produced by expression in a foreign host for medical healthcare applications in humans. Monoclonal antibodies (mAbs) are type of biologics that are designed to specifically bind to one target, with a purpose to inhibit a certain molecule that could be associated with a disease process. LÄS MER

  3. 2. Increased expression of therapeutic proteins by identification of 3'-UTRs from high expressing genes in CHO cells

    Master-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

    Författare :Alexander Westlund; [2019]
    Nyckelord :Mammalian cell; CHO; E. coli; protein expression; plasmid; transformation; Fc-fusion protein; flow cytometry; FACS; transfection; mRNA; UTR; gene engineering;

    Sammanfattning : Therapeutic proteins, a.k.a. biopharmaceuticals, are most commonly produced in expression systems derived from Chinese Hamstery Ovary (CHO) cells, thanks to great capacity of post-translational modifications like secretation, folding and glycosylation. LÄS MER

  4. 3. Increased expression of proteins in CHO cells by identification of signal peptides for improved secretion of translated proteins

    Master-uppsats, Linköpings universitet/Teknisk biologi

    Författare :Malin Strannermyr; [2018]
    Nyckelord :Chinese hamster ovary; signal peptide; protein expression; plasmid; library; gene; Fc-fusion; monoclonal antibody; E. coli; flow cytometry; transfection; transformation;

    Sammanfattning : Main purpose of this study was to increase protein expression in Chinese hamster ovary (CHO) cells by improving protein secretion of translated proteins. The goal was to find signal peptides from the screening of signal peptide libraries for improvement of protein secretion using a CHO-cell express selection system. LÄS MER

  5. 4. A platform for Chinese hamster ovary (CHO) cell genome engineering

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Jiten Doshi; [2016]
    Nyckelord :Chinese hamster; lentivirus; RMCE;

    Sammanfattning : The production of therapeutic recombinant proteins in heterologous systems has gained significance since the last decade. For recombinant proteins that require post-translational modifications (PTMs), mammalian systems are preferred. Chinese hamster ovary (CHO) cells are the mammalian cells of choice for production of recombinant proteins. LÄS MER

  7. 5. Investigation of Syndecan-1 Ectodomain Isolated from Chinese Hamster Ovary (CHO) Cell Culture Medium

    Kandidat-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi; Uppsala universitet/Obstetrik & gynekologi

    Författare :Daniel Croce; [2015]
    Nyckelord :proteoglycan; heparan sulfate; glycosaminoglycan; chondroitin sulfate; shedding;

    Sammanfattning : Syndecan-1 is a cell surface proteoglycan which participates in cell adhesion, differentiation, motility, morphogenesis and intracellular signaling. The two glycosaminoglycans heparan sulfate and chondroitin sulfate are covalently attached to the ectodomain of syndecan-1 via a tetra saccharide linkage sequence. LÄS MER