Sökning: "Chinese hamster"

Visar resultat 1 - 5 av 11 uppsatser innehållade orden Chinese hamster.

  1. 1. OPTIMIZATION OF CLONE SELECTION PLATFORM FOR PRODUCTION OF RECOMBINANT PROTEINS

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Basel Yousef; [2024]
    Nyckelord :;

    Sammanfattning : Background  Biologics are type of proteins that can be produced by expression in a foreign host for medical healthcare applications in humans. Monoclonal antibodies (mAbs) are type of biologics that are designed to specifically bind to one target, with a purpose to inhibit a certain molecule that could be associated with a disease process. LÄS MER

  3. 2. Development of specific host cell protein assays

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Anna Ivert Nordén; [2023]
    Nyckelord :Gyrolab; Immunoassay; HCP;

    Sammanfattning : The manufacturing and the development of biotherapeutic drugs involves the expression of biotherapeutic proteins in a host cell expression system followed by a purification process. Bioanalytical methods to measure impurities such as host cell proteins (HCPs) are needed to obtain a robust process and a safe drug according to regulatory requirements. LÄS MER

  4. 3. The roles of heparan sulfate in autophagy

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Asifa Khan; [2022]
    Nyckelord :Heparan sulfate; LC3-II; p62; Autophagy;

    Sammanfattning : Background: Autophagy is a lysosomal degradation process to eliminate damaged components from the cells. It also provides a survival mechanism in stressed conditions and limits growth processes. Heparan sulfate (HS) is a polysaccharide attached to different proteoglycans. LÄS MER

  5. 4. CUSTOMIZED QUANTIFICATION OF HOST CELL PROTEIN WITH BIO-LAYER INTERFEROMETRY

    Master-uppsats, Lunds universitet/Teknisk mikrobiologi

    Författare :Eva Sjöblom; [2020]
    Nyckelord :BLI; HCP; CHO; yeast; host cell protein; bio-layer interferometry; immunoassays; quantification; chinese hamster ovary; ELISA; applied microbiology; teknisk mikrobiologi; Biology and Life Sciences; Technology and Engineering;

    Sammanfattning : Host cell protein (HCP) is quantified in the purification steps of biopharmaceutical production and is part of ensuring the purity of the final drug product. The gold standard method for HCP quantification is enzyme-linked immunosorbent assay (ELISA), but a new approach is proposed here with fully customized bio-layer interferometry assays for yeast and chinese hamster ovary (CHO) cells. LÄS MER

  7. 5. Evaluation of Potential Cytotoxic and Genotoxic Effects of Propolis in CHO-K1 Cells Using an in vitro Version of the Micronucleus Assay

    Master-uppsats, Uppsala universitet/Institutionen för farmaceutisk biovetenskap

    Författare :Elina Rosenborg; [2020]
    Nyckelord :propolis; micronucleus assay; genotoxicity; cytotoxicity; CHO-K1 cells; mitomycin C;

    Sammanfattning : Background Evaluating potential genotoxicity of pharmaceutical drug candidates is important during drug development. A method that can be used for this purpose is the micronucleus assay (MN- assay) which can identify agents that induce chromosomal damage. LÄS MER