Sökning: "FRET assay"

Hittade 2 uppsatser innehållade orden FRET assay.

1. 1. In Vitro Assessment of Novel Compounds as Potential Pan-Coronavirus Therapeutics in SARS-CoV-2 and In Vitro Assessment of a Pan-Flavivirus Compound in Zika Virus

   Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

   Författare :Julia Berger; [2022]
   Nyckelord :Human coronavirus; active protease inhibitors; drug discovery; 3CLpro; Zika virus; flavivirus inhibitor; NS2B-NS3;

   Sammanfattning : Through the SARS-CoV-2 pandemic, it has become clear that the development of antivirals is essential for the health and wellbeing of the population. In this study, novel active site protease inhibitors against SARS-CoV-2 were tested for their inhibitory activity against the viral 3-Chymotrypsin like protease through the means of FRET based enzymatic assays. LÄS MER

3. 2. Bacterial Sortase A as a drug target

   Uppsats för yrkesexamina på avancerad nivå, Institutionen för biologisk grundutbildning

   Författare :Caroline Larsson; [2012]
   Nyckelord :Antibiotic resistance; Sortase A; Staphylococcus aureus; Gram-positive bacteria; inhibition; FRET assay; fibronectin-binding;

   Sammanfattning : Sortase A is a housekeeping enzyme of Gram-positive bacteria that catalyses the anchoring of surface proteins to the bacterial peptidoglycan. The enzyme works to establish an interaction between bacteria and host cells and is essential for pathogenesis. LÄS MER