Sökning: "gene cloning"

Visar resultat 1 - 5 av 37 uppsatser innehållade orden gene cloning.

  1. 1. Identifiering av promotorregionen för Cyt c Id1 samt undersökning av genuttrycket i närvarooch frånvaro av syre

    Kandidat-uppsats, Karlstads universitet/Institutionen för ingenjörs- och kemivetenskaper (from 2013)

    Författare :Slava Nabo; [2023]
    Nyckelord :Ideonella dechloratans; Cytochrome c; Electron transport chain; pBBRlMCS-2-lacZ reporter vector; Chlorate reductase; Ideonella dechloratans; Cytokrom c; Elektrontransportskedja; pBBRlMCS-2-lacZ reportervektor; Kloratreduktas;

    Sammanfattning : In this work, the identification of the promoter for c-cytochromes Cyt c Id-1 in Ideonella dechloratans has been investigated. Additionally, the study examined investigating whether gene expression is affected by the presence and absence of oxygen. LÄS MER

  3. 2. Uttryck av ett nytt rekombinant protein Cp149 (HBV-kapsidprotein) modifierat med TfR apical domän

    Kandidat-uppsats, Linnéuniversitetet/Institutionen för kemi och biomedicin (KOB)

    Författare :Hamida Noorzai; [2022]
    Nyckelord :Gene cloning; Recombinant DNA; Transformation; Protein expression; SDS-PAGE; Flow Cytometry; Genkloning; rekombinant DNA; Transformering; Proteinuttryck; SDS-PAGE; Flödescytometri;

    Sammanfattning : Hepatit-B är en leversjukdom som orsakas av hepatit-B-virus (HBV) vilket är en kapslad DNA-virus. Kapsidprotein (Cp) har stor betydelse i virusets livscykel exempelvis DNA-replikation, interaktion med värdceller och andra virala glykoproteiner. HBV, som många andra virus, tar sig in i cellen genom att binda till cellreceptorer. LÄS MER

  4. 3. Potential Application of Multiplex Automated Genome Engineering (MAGE) and One-Step Curing Plasmid System for Environmental Cambodian Enterobacterial Isolates

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Olivia Alexandra; [2021]
    Nyckelord :Antimicrobial resistance; plasmid curing; environmental isolates; recombineering; enterobacteria;

    Sammanfattning : Antimicrobial resistance (AMR) is concerning because it limits antimicrobial drug treatment options. AMR occurs by the overuse and misuse of antimicrobial drugs. In environmental settings, AMR can disseminate from places of high use, which leads to increased exposure to humans and animals. LÄS MER

  5. 4. Automated pipeline for processing fluorescence calcium imaging microscopy data and electrophysiological measurements from 3D brain organoids

    Master-uppsats, Lunds universitet/Examensarbeten i bioinformatik

    Författare :Mattis Knulst; [2021]
    Nyckelord :Biology and Life Sciences;

    Sammanfattning : Motivation: 3D brain organoids are biological models of human brain development from which a rich body of data can be extracted that allows for insights about neuronal dynamics. Several tools, such as fluorescence calcium imaging microscopy and electrophysiological recordings are being used to assess how 3D brain organoid dynamics recapitulate what is known from the human brain. LÄS MER

  7. 5. Methods for production of recombinant enzymes for collagen degradation

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Författare :Tea Eriksson; Elin Su; [2020]
    Nyckelord :Collagenase; Subtilisin; Collagen; Enzymatic hydrolysis; Enzymes; Collagen degradation; Enzyme production; Cloning; Gene optimization; Protein expression; Technology and Engineering;

    Sammanfattning : In this thesis eight enzymes have been identified with documented ability to degrade collagen or its hydrolyzed products. Collagen can be found in skin, bones, and connective tissue. Hydrolyzed collagen peptides are utilized in food, disease treatment, skincare and training supplements. LÄS MER