Sökning: "pGAP"

Hittade 2 uppsatser innehållade ordet pGAP.

1. 1. Hello P. pastoris! : The cultivatin and expression of proteins in the yeast Pichia pastoris

   Kandidat-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

   Författare :Håkansson Anja; Dalén Therese; Gröblacher Josefine; Göransson William; Jaksties Jonathan; Ortstad Nathalie; Lenkeit Gesser Pauline; [2022]
   Nyckelord :Affibody AB; Pichia pastoris; pAOX1; pAOX2; pFLD1; pGAP; cultivation; fermentation; glycosylation;

   Sammanfattning : Producing pharmaceuticals in Escherichia coli inevitably comes with an extensive purification process. This is because many of the native proteins of E. coli are immunogenic to humans, especially the heat and pH resistant endotoxins located in the membrane of E. coli. LÄS MER

3. 2. Signal peptide exchange for higher expression of Bjerkandera adusta Loosenin 1 in P. pastoris

   Kandidat-uppsats, SLU/Department of Molecular Sciences

   Författare :Linus Brinkenstråhle; [2018]
   Nyckelord :Loosenin; cellulose; signal peptide; expansin; Pichia pastoris; recombinant expression;

   Sammanfattning : The protein Loosenin 1 from the wood degrading fungus Bjerkandera adu-sta (BaLOOS1) has been shown to loosen up recalcitrant biomass and en-hance enzymatic saccharification. BaLOOS1 is the only loosenin protein that has been biochemically characterised so far. LÄS MER