Sökning: "Protein Purity"

Visar resultat 1 - 5 av 25 uppsatser innehållade orden Protein Purity.

  1. 1. Optimization of two high-performance size-exclusion chromatography methods with diode-array detection for protein quantification and purity assessment

    Master-uppsats, Lunds universitet/Centrum för analys och syntes

    Författare :Camilla Lane; [2023]
    Nyckelord :Method qualification; Protein concentration; Protein Purity; High Performance Size exclusion chromatography; Quality control; Analytical chemistry; Biology and Life Sciences;

    Sammanfattning : Background: There is a need to develop methods of determining concentration and purity for two new affinity ligands, protein T and protein H, in order to ensure the quality of an industrial production of said proteins. Aims: To develop a High Performance Size Exclusion Chromatography, HPSEC, method to determine concentration and purity of protein T and to improve an existing HPSEC method for concentration and purity determination of protein H that show selectivity, display linearity between peak area and injection amount and give repeatable results. LÄS MER

  3. 2. Protein Recovery from Alkaline Extracts: A Comparative Analysis of Isoelectric Precipitation and Ultrafiltration

    Master-uppsats, Lunds universitet/Livsmedelsteknik och nutrition (master)

    Författare :Anjay Sharma; [2023]
    Nyckelord :Rapeseed cake; Brewers spent grains; Protein recovery; Ultrafiltration; Alkaline extraction; Food engineering; Technology and Engineering;

    Sammanfattning : Proteins are one of three macro-components of a diet. An increasing demand for products that can be a complete protein source exists. The vulnerability of the current food system towards environmental impacts and the inability to keep up with the rising population has shifted the focus on valorising underutilised by-products of the food industry. LÄS MER

  4. 3. Comparison of purifying DARPins using conventional methods and self-cleaving affinity chromatography

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Biokemi

    Författare :Sofia Larsson; [2023]
    Nyckelord :;

    Sammanfattning : An important issue in pharmaceutical industry is to develop processes to purify proteins. Thereare many purification methods that can be used for this purpose, but all methods have differentconstraints. LÄS MER

  5. 4. Engineered imaging scaffolds for cryo-EM of small proteins of interest

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Oscar Friberg; [2022]
    Nyckelord :Protein structure determination; single-particle cryo-EM; imaging scaffold; size limitation problem; affibody; YgjG.;

    Sammanfattning : Strukturbestämning av proteiner är viktigt för att kunna förstå deras funktion och en snabbt utvecklande metod inom fältet är kryoelektronmikroskopi. Storleksbegränsningar förhindrar en bredare applikation av metoden eftersom små proteiner har för låg signal i förhållande till bakgrund för att kunna visualiseras som enstaka partiklar i elektronmiksoskopibilder. LÄS MER

  7. 5. Relation between αs1-casein, proteolytic activity and composition of milk from Swedish goats

    Master-uppsats, SLU/Department of Molecular Sciences

    Författare :Emma Larsson; [2021]
    Nyckelord :swedish goat milk; αs1-casein; total proteolysis; plasmin; plasminogen; spectrophotometry;

    Sammanfattning : In the recent decade, locally produced food has gained in interest among Swedish consumers. Consumer values for buying local food has to do with concerns about diversity, purity and authenticity of food as well as a desire to support rural areas. One type of product that has gained in interest is locally produced goat cheese. LÄS MER