Effect Of Different Exercise Modalities On Skeletal Muscleangiogenic Markers In Adolescent Males With Type 1Diabetes Mellitus

Sammanfattning: Introduction: Type 1 Diabetes Mellitus (T1DM) patients can start developing micro- and macrovascular complications during childhood and are known to have vascular impairments such asendothelial dysfunction, vessel stiffness and reduced capillary density. Angiogenesis is theformation of new blood vessels from existing ones and is well documented to occur in healthyindividuals in response to various exercise modalities. In T1DM, angiogenesis can however bealtered and studies on exercise induced angiogenesis in adolescents with T1DM are lacking.The aim of this study was to compare the effects of three different exercise modalities onmolecular markers of angiogenesis in skeletal muscle of adolescent males with T1DM.Materials and Methods: Eight adolescent males (17.5 ± 0.8 years) with T1DM duration >1yearattended four sessions, namely a control session followed by exercise tests (VO2max, 1RM) andthree randomized exercise sessions (Continuous Exercise (CE), Intermittent Exercise (IE) andStrength Exercise (SE)). One hour after exercise and control a muscle biopsy was taken from thevastus lateralis muscle. RNA was extracted followed by one colour microarray-based geneexpression analysis as well as enrichment analysis of differentially expressed genes forbiological processes and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways.Results: Biological Processes related to angiogenesis (blood vessel development, angiogenesis,blood vessel endothelial cell migration, cell migration involved in sprouting angiogenesis) werehighlighted following all three exercise modalities, with no exercise specific response beingobserved. A total 7 KEGG pathways were highlighted across all the conditions, with AGERAGEsignaling in diabetic complications as well as Fluid shear stress and atherosclerosispathways including angiogenesis.Conclusion: In skeletal muscle of adolescent males with T1DM, CE, IE and SE all elicited acuteangiogenic response and KEGG pathways that may provide some insight into interplay of otherfactors in angiogenic signaling in T1DM skeletal muscle.