Sökning: "limited proteolysis"

Visar resultat 1 - 5 av 6 uppsatser innehållade orden limited proteolysis.

  1. 1. Characterization of bovine milk from indigenous species from Sri Lanka

    Master-uppsats, SLU/Department of Molecular Sciences

    Författare :Jonathan Skager; [2022]
    Nyckelord :Milk; total proteolysis; protein profile; capillary electrophoresis; fluorescence; indigenous cattle; Jersey cattle; Friesian cattle; Sri Lanka;

    Sammanfattning : Sri Lanka is a country in south Asia that from the beginning of the 21st century, until 2016 almost doubled their quantity in milk import, this to meet their national market demand for dairy products. The country is divided into different agroecological zones, which are the up-country, mid-country, and the lowlands. LÄS MER

  3. 2. Isolation of the native chloroplast proteome from plant for identification of protein-metabolite interactions

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Linnéa Strandberg; [2021]
    Nyckelord :Chloroplast; metabolism; proteomics; Calvin cycle; Arabidopsis; Kloroplast; metabolism; proteomik; Calvincykel; Arabidopsis;

    Sammanfattning : För att kunna livnära en växande population behöver avkastningen på skördar öka. En lösning på dettaär att optimera plantornas fotosyntes, vilket innefattar förbättrad koldioxidfixering. För att lyckas meddet krävs kunskap i hur reglering av nyckelproteiner i kloroplasten går till. LÄS MER

  4. 3. Development of biophysical methods for characterization of PROTACs

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Biokemi

    Författare :Westlin Oskar; [2021]
    Nyckelord :;

    Sammanfattning : Targeting protein with the aim of ubiquitin-labelling for degradation is a new and upand coming field of drug discovery. Proteolysis targeting chimeras (PROTACs) areheterobifunctional linker molecules that connects the target molecule to an E3ubiquitin ligase, subsequent ubiquitination of the target protein initiates the proteindegradation by the proteosome system. LÄS MER

  5. 4. Development of an antibioticsresistancebased method fordirected evolution of proteases

    Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Författare :Vilma Lagebro; [2020]
    Nyckelord :Protease engineering; protease; directed evolution; TEVp; Amyloid beta; CAT;

    Sammanfattning : Proteases have a fundamental role in regulating diverse biological processes and are important in the biotechnological and medical fields. Therapeutic proteases have great potential but have been limited due to the lack of high throughput protein engineering methods. LÄS MER

  7. 5. Identification of metabolite-protein interactions among enzymes of the Calvin Cycle in a CO2-fixing bacterium

    Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Författare :Emil Sporre; [2020]
    Nyckelord :Cupriavidus necator; chemolithoautotroph; limited proteolysis; Calvin Cycle; carbon fixation; proteomics; allosteric regulation; metabolite-protein interactions.;

    Sammanfattning : The Calvin – Benson cycle is the most widespread metabolic pathway capable of fixing CO2 in nature and a target of very high interest to metabolic engineers worldwide. In this study, 12 metabolites (ATP, AMP, NADP, NADPH, 2PG, 3PGA, FBP, RuBP, PEP, AKG, Ac-CoA and phenylalanine) were tested for protein – metabolite interactions against the proteome of Cupriavidus necator (previously Ralstonia eutropha) in the hopes of finding potential examples of allosteric regulation of the Calvin – Benson cycle. LÄS MER