Effekt av ändrade centrifugeringsparametrar på rutinkemi- och koagulationsanalyser

Sammanfattning: To separate plasma/serum from blood cells, blood samples are centrifuged. Currently, there is no standard centrifugation protocol available. However, there are several centrifugation recommendations from the tube manufacturers. The aim of this study is to evaluate centrifugation program with a shorter centrifugation time and higher centrifugal force and how it affects measured analyte concentrations. 120 sample pairs were collected in lithium-heparin tubes and sodium citrate tubes from 120 participants. One sample of each pair was then centrifuged with reference program (2000g, 10min) for routine chemistry samples and (2500g, 15min) for coagulation samples. The other sample of each pair was centrifuged with programs (3000g, 7min). The statistical analysis was performed using the Student’s t-test and the Holm-Bonferroni method. P-value less than 0.05 was considered statistically significant.There was no significant difference between data from BD tubes and Greiner tubes, therefore the corresponding data from both groups were merged. No statistically significant difference was observed for the following parameters; Cobalamin (B12), High-density lipoprotein (HDL), Lactate dehydrogenase (LD), Phosphate, Triglycerides (TG), Thyroid stimulating hormone (TSH), Activated partial thromboplastin time (APTT), Fibrinogen (Fib) and Prothrombin complex PK (INRR). However, the statistical analysis showed a significant difference regarding potassium (K). Centrifugation program (3000g, 7min) can replace the programs (2000g, 10min) and (2500g, 15min) which are recommended by the tube manufacturers BD & Greiner, with the exception of Potassium which requires more research. A trace of increasing hemolysis, still within acceptable limits, was detected in samples centrifuged with programs (3000g, 7min). The centrifugation programs with a shorter time or less acceleration is preferred.