Sökning: "Single-cell"

Visar resultat 11 - 15 av 99 uppsatser innehållade ordet Single-cell.

  1. 11. Image-Based Classification Solutions for Robust Automated Molecular Biology Labs

    Master-uppsats, KTH/Medicinteknik och hälsosystem

    Författare :Arnold Teo; [2023]
    Nyckelord :Neural networks; Deep learning; Transfer learning; TensorFlow; Single-cell genomics; Image classification; Neurala nätverk; Djupinlärning; Överförd inlärning; TensorFlow; Enskild cellgenomik; Bildklassificering;

    Sammanfattning : Single-cell genomics (SCG) are methods for investigating heterogeneity between biological cells, among these is Smart-seq which sequences from RNA molecules. A more recent version of this method is Smart-seq3xpress which is currently in the process of being automated by the Sandberg lab at Karolinska Institutet. LÄS MER

  3. 12. Identifying cell type-specific proliferation signatures in spatial transcriptomics data and inferring interactions driving tumour growth

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Neuroonkologi och neurodegeneration

    Författare :Felix Wærn; [2023]
    Nyckelord :spatial transcriptomics; breast cancer; dimensionality reduction; bioinformatics; deconvolution;

    Sammanfattning : Cancer is a dangerous disease caused by mutations in the host's genome that makes the cells proliferateuncontrollably and disrupts bodily functions. The immune system tries to prevent this, but tumours have methods ofdisrupting the immune system's ability to combat the cancer. LÄS MER

  4. 13. Single-cell transcriptomic and antibody-based proteomic analysis of Sertoli cell-specific markers in human testis

    Kandidat-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Katharina Stanzel; [2023]
    Nyckelord :;

    Sammanfattning : The testis is a complex organ responsible for sperm production and hormone synthesis. Spermatogenesis, the process of sperm formation, occurs in the seminiferous tubules and involves the coordinated development of germ cells with the support of Sertoli cells. LÄS MER

  5. 14. Automated Quantitative Phase Imaging Pipeline : for Monitoring Bacterial Cell Growth under Chemical Influence

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Avdelningen Vi3

    Författare :Samuel Zargani; [2023]
    Nyckelord :image analysis; QPI; Quantitative Phase Imaging; QLSI; kvantitativ fasavbildning; Bildanalys;

    Sammanfattning : Bacteria have a vital role in ecosystems and the human health. However, the effect of environmental pollution on them at the single-cell level are still not well understood. Label-free imaging has become increasingly important within science. It has allowed for the visualization of cells without the use of molecular tools or labels. LÄS MER

  7. 15. Active dielectrophoretic trapping for deterministic single-cell encapsulation in droplet microfluidics

    Master-uppsats, KTH/Skolan för elektroteknik och datavetenskap (EECS)

    Författare :Prasanna Surana; [2023]
    Nyckelord :negative dielectrophoresis; droplet microfluidics; low conductivity medium; cell viability; COMSOL electric field simulation; joule heating; Rhodamine B temperature measurement; microfluidic chip cleaning; negativ dielektrofores; droppmikrofluidik; medium med låg konduktivitet; cellviabilitet; COMSOL elektrisk fältsimulering; jouleuppvärmning; Rhodamine B temperaturmätning; mikrofluidisk chiprengöring;

    Sammanfattning : The research work focuses on optimizing various parameters for controlling cells using negative dielectrophoresis and entrapping them in droplet microfluidics. This is achieved by developing a conductivity medium, combining CytoRecovery, BSA, and EDTA, to maintain a steady count of single cells with good viability over an extended period. LÄS MER