Sökning: "hpRNA"

Hittade 1 uppsats innehållade ordet hpRNA.

  1. 1. Construction of hpRNA expression vector for silencing a gene in Rhizopus oryzae

    Magister-uppsats, Högskolan i Borås/Institutionen Ingenjörshögskolan

    Författare :Kiran Kumar Penmatsa; Bharat Balu; [2012]
    Nyckelord :RNA interference; hpRNA; Silencing vector construction; Rhizopus oryzae; Ethanol; Lactic acid;

    Sammanfattning : Depending on the previous research on LDHA gene silencing in Rhizopus oryzae CCUG 28959 through introduction of siRNA, a integral vector was constructed by inserting two copies of LDHA gene (by PCR cloning) in a fashion that it can express hpRNA in the transformed fungi, which will trigger the post transcriptional degradation of targeted mRNA through RNA degradation pathway which is known to be quelling in fungi.The vector was successfully designed with the LDHA gene, transformed in to the host organism, and also transferred to its progeny. LÄS MER