Sökning: "DNA purification"

Visar resultat 1 - 5 av 36 uppsatser innehållade orden DNA purification.

1. 1. Method development of magnetic cell isolation and DNA extraction of small cell populations from Ficoll-separated hematopoietic cells

   Kandidat-uppsats, Uppsala universitet/Institutionen för medicinsk cellbiologi

   Författare :Dominika Debowska; [2023]
   Nyckelord :Clonal haematopoiesis of indeterminate potential CHIP ; hematopoietic stem cell; automated cell separation; silica-membrane-based nucleic acid purification.;

   Sammanfattning : Clonal haematopoiesis of indeterminate potential, or CHIP are a family of mutations present in the general population. CHIP-mutations are prevalent in the haematopoietic stem cells and in the more mature cell populations, T-lymphocytes, B-lymphocytes and myeloid cells (CD3+, CD19+ and CD33+ cells) in blood. LÄS MER

3. 2. Navigating Large-Scale Plasmid DNA Purification : A Recommendation of Current and Future Downstream Purification Solutions

   Kandidat-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

   Författare :Matilda Eriksson; Alva Wells; Maria Frey; Lisa Johansson; Gabriel Pettersson; David Sjöberg; [2023]
   Nyckelord :Plasmid purification; pDNA; large-scale chromatography;

   Sammanfattning : Previous small-scale methods for plasmid DNA (pDNA) purification fail to meet theindustry’s demand for sufficient quantities. Greater volumes of bacterial lysates are a consequence of larger volumetric fermentations and traditional large-scale down-stream purification processes have some disadvantages and limitations. LÄS MER

4. 3. Utvärdering av BioFire Joint Infection Panel för mikrobiologisk diagnostik av ledvätska

   Kandidat-uppsats, Linnéuniversitetet/Institutionen för kemi och biomedicin (KOB)

   Författare :Tilda Bengtsson; [2023]
   Nyckelord :Synovial fluid; septic arthritis; multiplex PCR; FilmArray; BioFire Joint Infection Panel; Ledvätska; septisk artrit; multiplex PCR; FilmArray; BioFire Joint Infection Panel;

   Sammanfattning : En bakteriell ledinfektion kallas för septisk artrit och utan snabb behandling kan tillståndet leda till irreversibla ledskador och flera allvarliga komplikationer. Snabb diagnostik är viktigt för en god prognos och med nuvarande metod för odling av ledvätska tar det upp till fem dygn för detektion av mikroorganismer. LÄS MER

5. 4. Design and production of adeno-associated virus vectors for imaging mitochondrial networks in the brain

   Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för biologisk grundutbildning

   Författare :Elnaz Samadian Zad; [2023]
   Nyckelord :adeno-associated virus; mitochondria; mitochondrial networks; imaging; fluorescent protein; synapsin promoter; human embryonic kidney cells; organoids;

   Sammanfattning : Mitochondria are dynamic organelles that function in a complex interconnected network within the cell. Neurons are sensitive and highly energy demanding cells in the brain which require a functioning mitochondrial network that is able to provide ATP and modulate calcium. LÄS MER

7. 5. Towards time-resolved cryo-EM of SARS-CoV-2 replication-transcription complex and Staphylococcus aureus DNA gyrase

   Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

   Författare :Anna Králová; [2023]
   Nyckelord :time-resolved cryo-EM; DNA gyrase; SARS-CoV-2; replication-transcription complex; protein purification;

   Sammanfattning : Time-resolved cryo-EM has already provided ground-breaking discoveries in various fields, including structural biology, biochemistry, and drug development. Compared to traditional structural biology methods where mostly stabilized conformations are reconstructed, the main advantage of time-resolved cryo-EM is its ability to capture dynamic processes in biological samples at near-atomic resolution, which allows for studying biological structures as they change and interact in real-time. LÄS MER