Sökning: "Fluorescence Resonance Energy Transfer FRET"

Visar resultat 1 - 5 av 6 uppsatser innehållade orden Fluorescence Resonance Energy Transfer FRET.

  1. 1. Co-evolutional anaylsis of the Na+,K+-ATPase’s β-subunit dimerization

    Master-uppsats, KTH/Tillämpad fysik

    Författare :Sebastian Bauer; [2023]
    Nyckelord :Na ; K -ATPase NKA ; Co-evolution; Dimerization; Direct coupling analysis DCA ; Molecular dynamic MD simulation; Contact filtering; Mapping; Na ; K -ATPas NKA ; Samevolution; Dimerisering; Direkt kopplingsanalys DKA ; Molekylär dynamisk MD simulering; Kontaktfiltrering; Kartläggning;

    Sammanfattning : Does the active membrane transporter, Na+,K+-ATPase dimerize? If it does, whatis the functional benefit? Does it increase or decrease the turnover rate? Theseare still unanswered questions and current research topics. Previous studies havedemonstrated dimerizations in closely related proteins of the P-type ATPase family. LÄS MER

  3. 2. Building a Fluorescence Correlation Spectroscopy (FCS) instrument followed bytesting the boundaries of FRET-FCS

    Kandidat-uppsats, KTH/Skolan för teknikvetenskap (SCI)

    Författare :Isabell Weinberg-Krakowski; Evelina Stenseth; [2021]
    Nyckelord :;

    Sammanfattning : Understanding biological systems oftentimes requires mapping the behaviors of biomolecules on small scales such as single proteins or nucleic acids. Fluorescence Correlation Spectroscopy (FCS) is a group of particularly noninvasive and sensitive fluorescence-­based techniques that can be used for this purpose. LÄS MER

  4. 3. Study of co-translational folding of E. coli dihydrofolate reductase using fluorescence resonance energy transfer (FRET)

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Aswathy Kallazhi; [2018]
    Nyckelord :FRET; tRNA; co-translational folding; protein folding; DHFR; fluorescence; aminoacylation;

    Sammanfattning : In prokaryotes, protein synthesis and folding are often coupled, and the protein begins to fold from the N-terminus as it is being synthesized. It has been hypothesised that there could be kinetic coupling of the speed of translation and the folding, which means that an altered rate of synthesis can cause a possible misfolding of the protein. LÄS MER

  5. 4. In vivo assessment of focal adhesion kinase (FAK) activity in breast cancer cells using fluorescence resonance energy transfer (FRET) sensor and confocal laser scanning microscope (CLSM)

    Magister-uppsats, Högskolan i Skövde/Institutionen för hälsa och lärande

    Författare :Sujithera Haridoss; [2015]
    Nyckelord :;

    Sammanfattning : Focal Adhesion Kinase (FAK) is essential for cell migration and plays an important role in tumor metastasis. However, the complex intermolecular and intramolecular interactions that regulate FAK activity at the focal adhesion remain unresolved. LÄS MER

  7. 5. DNA chips with conjugated polyelectrolytes as fluorophore in fluorescence amplification mode

    Uppsats för yrkesexamina på grundnivå, Institutionen för fysik, kemi och biologi

    Författare :Karin Magnusson; [2008]
    Nyckelord :Soft lithography; conjugated polyelectrolyte CPE ; DNA-chip; fluorescence microscope; Fluorescence Resonance Energy Transfer FRET ;

    Sammanfattning : The aim of this diploma work is to improve selectivity and sensitivity in DNA-chips by utilizing fluorescence resonance energy transfer (FRET) between conjugated polyelectrolytes (CPEs) and fluorophores. Leclerc and co-workers have presented successful results from studies of super FRET between fluorophore tagged DNA and a CPE during hybridisation of the double strand. LÄS MER