Stress response in cancer cell lines

Sammanfattning: Globally, the cases of cancer have been on the rise. This has led to increasing research to find a lasting solution to carcinogenesis. The increase in cancer cases can be due to a change in people’s lifestyles, such as diet and exercise routines which have changed for the worse. In most farms, chemicals as pesticides are added to plants to fasten their growth. These chemicals are carcinogenic, contributing to the increased number of cancer cases worldwide. The objective of this research was to observe the cell response of brain, pancreatic, and breast cancer cells to different cortisol levels/concentrations. The three cell lines of interest in this research and present in the mentioned types of cancer are T-47D, PANC-1, and T98G. Their dynamics and roles are identified in this study. Cortisol excretion concentrations during stress and cancer growth are also monitored and compared. Additionally, the study solves the unpredictability of the impacts of stress on the three cancer types. The three experimental setups in this study were as follows: 1. Breast cancer cells, obtained from a 54 year old woman with metastatic carcinoma. 2. Brain cancer cells, obtained from a 61 year old female with neuroblastoma. 3. Pancreatic cancer cells, obtained from a 56 year old male with metastatic carcinoma. The cells in the above setups were treated with cortisol in order to see what effect this induced stress has on cell growth. The findings of the study concluded higher cortisol concentration increased cancer growth and spread within the body. This was that all three cancer cell types saw an increased effect of cortisol at either 24 hours or 48 hours at a specific concentration, which highlights that cortisol has an important role to play in cancer cells viability.