Sökning: "Modified oligonucleotides"

Hittade 5 uppsatser innehållade orden Modified oligonucleotides.

  1. 1. Non-coding RNAs as therapeutic agents : The future of therapy

    Kandidat-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Hederoth Gustaf; Lyngå Olivia; Rudqvist Olivia; Jeppsson Rebecka; Hogolof Saida; Svanberg Sara; Kauppinen Veera; [2022]
    Nyckelord :Non-coding RNA; Oligonucleotide therapeutics;

    Sammanfattning : Non-coding RNA (ncRNA) therapeutics are based on short oligonucleotides, both naturally occurring and artificial, which target RNA in a site-specific way to modulate gene expression. As of today, 12 synthetically produced ncRNA-based drugs are available on the market in the US and Europe, and there is a possibility of more to be approved in the near future. LÄS MER

  3. 2. Comprehensive Study on Aptamers and Aptamer-based Assays

    Kandidat-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Axel Truedson; Márta Sundström; Christoffer Eriksson; Andreas Bergfeldt; Matilda Jägare Lindvall; Caroline Normann; [2022]
    Nyckelord :Aptamer; Biotechnology; Detection method; Biosensor; Molecular recognition; Antibodies; Aptamerer; Bioteknik; Detektionsmetod; Biosensor; Molekylär igenkänning; Antikroppar;

    Sammanfattning : Antibodies are the gold standard molecular recognition elements and a cornerstone of molecular biology. They are used in immunoassays to precisely measure a specific analyte, but certain targets are especially challenging. Difficult targets include small molecules and molecules that do not induce an immune response. LÄS MER

  4. 3. Preparative chromatographyfor modified oligonucleotides : Method development for modified oligonucleotides, fromanalytical to preparative chromatography

    Master-uppsats, Karlstads universitet

    Författare :Rebecka Jasinski; [2021]
    Nyckelord :oligonucleotides; preparative chromatography; scaling-up; ion-pair chromatography; dibutylamine; oligonukleotider; preparativ kromatografi; uppskalning; jonparskromatografi; dibutylamin;

    Sammanfattning : Synthetic oligonucleotides, which are short strings of DNA or RNA, are a grooving area of importance for the pharmaceutical industry and for companies that manufacture diagnostic components. The manufacturing process of synthetic oligonucleotides involves many complex processes that use separation and purification techniques like ion-exchange chromatography, ion-pair reversed phase chromatography and ultra-performance liquid chromatography. LÄS MER

  5. 4. The impact of various chromatographic conditions on the separation of modified and unmodified oligonucleotides

    Kandidat-uppsats, Karlstads universitet

    Författare :Lewis Frazer; [2021]
    Nyckelord :Oligonucleotide; Modified oligonucleotides; Ion-pair chromatography; Chromatography; Analytical chemistry; TEAA; DBA; Chemistry; Oligonukleotid; Modifierade oligonukleotider; Jonpars kromatografi; Kromatografi; Analytisk kemi; TEAA; DBA; Kemi;

    Sammanfattning : In this study, the effects of certain chromatographic conditions on various modified and unmodified oligonucleotides were investigated. At the forefront of this study was the investigation of a new Ion-pair Reversed-phase liquid chromatography (IP-RPLC) method, that had the potential to replace a previously established triethylammonium acetate (TEAA) IP-RPLC method developed for oligonucleotide separations. LÄS MER

  7. 5. Studies towards a general method for attachment of a nuclear import signal. Stabilization of the m3G-Cap.

    Master-uppsats, Akademin för hållbar samhälls- och teknikutveckling

    Författare :Mattias Lindvall; [2010]
    Nyckelord :m3G-cap; nuclear transport; Duchennes Muscular Dystrophy; Guanosine triphosphate analouges;

    Sammanfattning : A synthetic pathway towards the cap-structure of 2,2,7-trimethylguanosine containing a methylene modified triphosphate bridge have been investigated. The modification to the triphosphate bridge is hoped to slow down cap degradation and give the connected  oligunucleotide an increased lifetime. LÄS MER