The role of Fragile-X mental retardation-related protein 1 in Human Adenovirus 5 infection

Sammanfattning: The Fragile X-related mental retardation 1 (FXR1) is an N6-Methyladenosine reader involved in mRNAs metabolism like mRNA splicing, stability, transport, and miRNA regulation. It is also important in transcription, cell proliferation, differentiation, translation, polysome assembly and stress granule assembly. The protein is present in all eukaryotic cells, but so far it has been specifically essential for correct neural function. Until now, FXR1 has not been investigated in the concept of Human Adenovirus infection but we have observed an upregulation of FXR1 during the late phase of the Human Adenovirus 5 (HAdV-5) infection and an upregulation of some late HAdV-5 proteins in HeLa cells overexpressing FXR1. Our results furthermore showed that a FXR1 knockdown resulted in a reduced level of some HAdV-5 proteins at the same time as HAdV-5 mRNA were stabilized, indicating that FXR1 might be involved in translation of HAdV-5 late genes. Further investigation of the mechanism behind FXR1 mediated translation, a Death-associated protein 5 (DAP5) was founded to have an overall effect on the translation of HAdV-5 late proteins.