Sökning: "Anton Elfstrand"

Hittade 1 uppsats innehållade orden Anton Elfstrand.

  1. 1. Production and analysis of novel disulfide variants of Subtilisin Carlsberg

    Master-uppsats, Linköpings universitet/Kemi

    Författare :Anton Elfstrand; [2023]
    Nyckelord :Subtilisin Carlsberg; Disulfide; Protein Engineering; Kinetic Stability;

    Sammanfattning : Protein engineering has been used to alter the stability of proteins for several decades withmuch success, one approach being to introduce two cysteine residues that together form adisulfide bridge. The disulfide bridge can increase the Gibbs free energy of the transitionstate, thus increasing energy difference between the folded state and the unfolding transitionstate, leading to increased kinetic stability of the protein. LÄS MER