Sökning: "Subtilisin Carlsberg"

Hittade 2 uppsatser innehållade orden Subtilisin Carlsberg.

1. 1. Production and analysis of novel disulfide variants of Subtilisin Carlsberg

   Master-uppsats, Linköpings universitet/Kemi

   Författare :Anton Elfstrand; [2023]
   Nyckelord :Subtilisin Carlsberg; Disulfide; Protein Engineering; Kinetic Stability;

   Sammanfattning : Protein engineering has been used to alter the stability of proteins for several decades withmuch success, one approach being to introduce two cysteine residues that together form adisulfide bridge. The disulfide bridge can increase the Gibbs free energy of the transitionstate, thus increasing energy difference between the folded state and the unfolding transitionstate, leading to increased kinetic stability of the protein. LÄS MER

3. 2. Methods for production of recombinant enzymes for collagen degradation

   Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

   Författare :Tea Eriksson; Elin Su; [2020]
   Nyckelord :Collagenase; Subtilisin; Collagen; Enzymatic hydrolysis; Enzymes; Collagen degradation; Enzyme production; Cloning; Gene optimization; Protein expression; Technology and Engineering;

   Sammanfattning : In this thesis eight enzymes have been identified with documented ability to degrade collagen or its hydrolyzed products. Collagen can be found in skin, bones, and connective tissue. Hydrolyzed collagen peptides are utilized in food, disease treatment, skincare and training supplements. LÄS MER