Identification of protein-interaction partners of ZBED6

Sammanfattning: ZBED6 was recently discovered as a novel transcriptional repressor of IGF2 because a mutation disrupting one of its binding sites in porcine IGF2 intron 3 leads to greater postnatal IGF2 expression in skeletal muscle and makes pig grow more muscle. ZBED6 is unique to placental mammals and derived from domesticated DNA transposon. However, the mechanism of ZBED6-mediated transcriptional regulation is still unknown. The study of the protein interacting partners of ZBED6 is a straightforward way to help us answer the question. By using the cells with stable overexpression of ZBED6, we perform one-step pull down assay and tandem affinity purification. A protein complex, SFPQ-Nono, is identified as the protein-interaction partners of ZBED6 by using mass spectrometry.